SPORL Pretreatment Spent Liquors Enhance the Enzymatic Hydrolysis of Cellulose and Ethanol Production from Glucose,Energy & Fuels

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SPORL Pretreatment Spent Liquors Enhance the Enzymatic Hydrolysis of Cellulose and Ethanol Production from Glucose
Energy & Fuels ( IF 5.2 ) Pub Date : 2018-06-19 00:00:00 , DOI: 10.1021/acs.energyfuels.8b00864
Haifeng Zhou _{1,

2} , Roland Gleisner ₂ , J.Y. Zhu ₂ , Yuanyu Tian _{1,

3} , Yingyun Qiao ₃

Affiliation

This study investigated the recycle utilization of SPORL pretreatment spent liquor. Three lignosulfonates (LSs) were purified from the spent liquor of SPORL pretreated Beetle-killed lodgepole pine (BKLP), Poplar NE222 (NE222), and Douglas-fir (FS10). The structural characterization showed that the apparent molecular mass and sulfur content of NE222-LS were lowest, but the phenolic group content was highest. FS10-LS, from a pH profiling SPORL pretreatment, had the highest apparent molecular mass but medium sulfur and phenolic group content. The spectral analyses exhibited that the guaiacyl unit was the main structure in BKLP and FS10 LSs, while NE222-LS mainly contained both guaiacyl and syringyl units. Both LSs and SPORL pretreatment spent liquors were used as additives to enzymatic hydrolysis of Whatman paper and ethanol production from glucose. LSs and liquors, from SPORL pretreated BKLP and NE222, could obviously enhance the enzymatic saccharification. Nevertheless, LS and liquor from SPORL pretreated FS10 presented a slight negative effect on enzymatic saccharification. All LSs and liquors with low concentration exhibited no inhibition on ethanol fermentation from glucose. When whole spent liquors without any detoxification were applied to prepare the fermentation medium with an initial glucose concentration of 100 g/L, the ethanol yield was almost the same as the control for BKLP and FS10 liquors. Nevertheless, the whole NE222 liquor without detoxification inhibited ethanol production thoroughly.

中文翻译：

SPORL预处理用过的白酒可增强纤维素的酶促水解作用，并从葡萄糖生产乙醇

这项研究调查了SPORL预处理废液的循环利用。从SPORL预处理的甲壳虫杀死的寄主松（BKLP），白杨NE222（NE222）和花旗松（FS10）的废液中纯化了三种木质素磺酸盐（LSs）。结构表征表明，NE222-LS的表观分子量和硫含量最低，而酚基含量最高。通过pH曲线SPORL预处理得到的FS10-LS具有最高的表观分子量，但具有中等的硫和酚基含量。光谱分析表明，愈创木基单元是BKLP和FS10 LS中的主要结构，而NE222-LS主要包含愈创木基和丁香基单元。LS和SPORL预处理废液均用作Whatman纸的酶促水解和葡萄糖生产乙醇的添加剂。来自SPORL预处理的BKLP和NE222的LSs和白酒可以明显增强酶促糖化作用。然而，来自SPORL预处理的FS10的LS和液体对酶促糖化反应有轻微的负面影响。所有低浓度的LS和液体均不抑制葡萄糖发酵乙醇。当将没有排毒的全废液用于制备初始葡萄糖浓度为100 g / L的发酵培养基时，乙醇收率几乎与BKLP和FS10液的对照相同。尽管如此，整个NE222液体没有解毒，仍能彻底抑制乙醇的产生。用SPORL预处理的FS10制成的LS和酒液对酶促糖化反应有轻微的负面影响。所有低浓度的LS和液体均不抑制葡萄糖发酵乙醇。当将没有排毒的全废液用于制备初始葡萄糖浓度为100 g / L的发酵培养基时，乙醇收率几乎与BKLP和FS10液的对照相同。尽管如此，整个NE222液体没有解毒，仍能彻底抑制乙醇的产生。用SPORL预处理的FS10制成的LS和酒液对酶促糖化反应有轻微的负面影响。所有低浓度的LS和液体均不抑制葡萄糖发酵乙醇。当将没有排毒的全废液用于制备初始葡萄糖浓度为100 g / L的发酵培养基时，乙醇收率几乎与BKLP和FS10液的对照相同。尽管如此，整个NE222液体没有解毒，仍能彻底抑制乙醇的产生。乙醇的产量几乎与BKLP和FS10酒的对照品相同。尽管如此，整个NE222液体没有解毒，仍能彻底抑制乙醇的产生。乙醇的产量几乎与BKLP和FS10酒的对照相同。尽管如此，整个NE222液体没有解毒，仍能彻底抑制乙醇的产生。

更新日期：2018-06-19

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