Short-wavelength visible light (blue light: 400–500 nm) has lethal effects on various insects, such as fruit flies, mosquitoes, and flour beetles. However, the most toxic wavelengths of blue light might differ across developmental stages. Here, we investigate how the toxicity of blue light changes with the developmental stages of an insect by irradiating Drosophila melanogaster with different wavelengths of blue light. Specifically, the lethal effect on eggs increased at shorter light wavelengths (i.e., toward 405 nm). In contrast, wavelengths from 405 to 466 nm had similar lethal effects on larvae. A wavelength of 466 nm had the strongest lethal effect on pupae; however, mortality declined as pupae grew. A wavelength of 417 nm was the most harmful to adults at low photon flux density, while 466 nm was the most harmful to adults at high photon flux density. These findings suggest that, as the morphology of D. melanogaster changes with growth, the most harmful wavelength also changes. In addition, our results indicated that reactive oxygen species influence the lethal effect of blue light. Our findings show that blue light irradiation could be used as an effective pest control method by adjusting the wavelength to target specific developmental stages.

短波可见光（蓝光：400-500 nm）对各种昆虫（如果蝇，蚊子和甲虫）具有致命作用。但是，蓝光的最具毒性的波长在整个发育阶段可能会有所不同。在这里，我们研究通过照射果蝇（Drosophila melanogaster），蓝光的毒性如何随着昆虫的发育阶段而变化。具有不同波长的蓝光。具体而言，对鸡蛋的致死作用在较短的光波长下（即朝405 nm处）增加。相反，从405到466 nm的波长对幼虫具有相似的致死作用。466 nm的波长对up具有最强的致死作用。然而，死亡率随着as的生长而下降。在低光子通量密度下，波长417 nm对成年人危害最大，而在高光子通量密度下，466 nm波长对成年人危害最大。这些发现表明，作为D的形态。忧郁症随着生长而变化，最有害的波长也会变化。此外，我们的结果表明，活性氧会影响蓝光的致死作用。我们的发现表明，通过调节波长以针对特定的发育阶段，可以将蓝光辐照用作有效的害虫防治方法。