Short-wavelength visible light (blue light: 400–500 nm) has lethal effects on various insects, such as fruit flies, mosquitoes, and flour beetles. However, the most toxic wavelengths of blue light might differ across developmental stages. Here, we investigate how the toxicity of blue light changes with the developmental stages of an insect by irradiating Drosophila melanogaster with different wavelengths of blue light. Specifically, the lethal effect on eggs increased at shorter light wavelengths (i.e., toward 405 nm). In contrast, wavelengths from 405 to 466 nm had similar lethal effects on larvae. A wavelength of 466 nm had the strongest lethal effect on pupae; however, mortality declined as pupae grew. A wavelength of 417 nm was the most harmful to adults at low photon flux density, while 466 nm was the most harmful to adults at high photon flux density. These findings suggest that, as the morphology of D. melanogaster changes with growth, the most harmful wavelength also changes. In addition, our results indicated that reactive oxygen species influence the lethal effect of blue light. Our findings show that blue light irradiation could be used as an effective pest control method by adjusting the wavelength to target specific developmental stages.

短波长可见光（蓝光：400-500 nm）对多种昆虫具有致命作用，如果蝇、蚊子和面粉甲虫。然而，蓝光中毒性最强的波长可能因发育阶段而异。在这里，我们通过用不同波长的蓝光照射果蝇来研究蓝光的毒性如何随着昆虫的发育阶段而变化。具体来说，较短的光波长（即接近 405 nm）对鸡蛋的致死作用会增加。相比之下，405 至 466 nm 的波长对幼虫具有类似的致死作用。 466 nm波长对蛹的致死作用最强；然而，随着蛹的生长，死亡率下降。 417 nm 波长在低光子通量密度下对成虫危害最大，而 466 nm 波长在高光子通量密度下对成虫危害最大。这些发现表明，随着D的形态。黑腹果蝇随着生长而变化，最有害的波长也会变化。此外，我们的结果表明活性氧会影响蓝光的致死效果。我们的研究结果表明，通过调整波长以针对特定的发育阶段，蓝光照射可以用作有效的害虫控制方法。