Thiacloprid is an insecticide belonging to the family of neonicotinoids, substances initially underestimated for their potential adverse effects, that they may manifest in the long term leading to an extensive use. The objective of this study was to evaluate the effect at increasing concentrations of thiacloprid on chick embryos development. The research was carried out on 75 fertile eggs of Gallus gallus domesticus. The eggs were opened after 10, 15 and 20 days of incubation and in treated embryos were observed developmental alterations, growth retardation, limbs defects and ectopia viscerale. The histological analysis showed hepatic steatosis and haemorrhages both in the liver and in the lungs. Moreover, the immunohistochemical analysis performed on the liver sections showed a strong positivity only for the erythrocytes to the anti-CYP1A antibody. Thiacloprid exposure increases the risks of teratogenic effects especially at the higher doses tested, therefore its use should be more controlled and limited. Since the literature on the topic is lacking, then the human health impacts resulting from neonicotinoids exposure is not yet fully understood, and, our data will be helpful to allow the assessment of an oral reference dose and health risk characterization.

噻虫啉是一种新烟碱类杀虫剂，最初被低估了它们的潜在不良作用，因此可能会长期导致广泛使用。这项研究的目的是评估噻虫啉浓度增加对雏鸡胚胎发育的影响。这项研究是在75个肥沃的鸡蛋进行鸡内金。孵化10、15和20天后打开卵，在处理过的胚胎中观察到发育变化，生长迟缓，四肢缺损和内脏异样。组织学分析显示肝和肺均发生肝脂肪变性和出血。此外，在肝切片上进行的免疫组织化学分析显示，仅对于抗CYP1A抗体的红细胞具有强阳性。噻虫啉的暴露增加了致畸作用的风险，尤其是在较高的测试剂量下，因此应更加控制和限制其使用。由于缺乏有关该主题的文献，因此尚未充分了解新烟碱类药物暴露对人体健康的影响，我们的数据将有助于评估口服参考剂量和健康风险特征。