Liquid crystalline hydrogels (LCGs) with layer structures and oriented pores were created using sacran which is a cyanobacterial heteropolysaccharide possessing functional sulfate, carboxylate, and amide groups in common with glycosaminoglycan. The LCG biocompatibility with L929 mouse fibroblasts was confirmed under the appropriate conditions. Enhanced growth and proliferation of L929 cells without exhibiting any toxicity were confirmed. The water contact angle and protein adsorption ability on the LCG were well-controlled by the cross-linking degree. Additionally, fibroblasts were finely oriented on the LCG side face where layer edges made a striped morphology on its surface, whereas the flat top faces of the LCG did not induce any specific cell orientation.

中文翻译：

---

蓝藻液晶凝胶上细胞方向的表面选择性控制。

使用萨克兰创建了具有层状结构和定向孔的液晶水凝胶（LCG），萨克兰是一种蓝藻杂多糖，具有与糖胺聚糖相同的功能性硫酸盐、羧酸盐和酰胺基团。在适当的条件下证实了LCG与L929小鼠成纤维细胞的生物相容性。证实了 L929 细胞的生长和增殖增强，且没有表现出任何毒性。LCG 上的水接触角和蛋白质吸附能力通过交联度得到很好的控制。此外，成纤维细胞在 LCG 侧面上精细定向，其中层边缘在其表面上形成条纹形态，而 LCG 的平坦顶面不会诱导任何特定的细胞定向。