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ZOLA-3D allows flexible 3D localization microscopy over an adjustable axial range.
Nature Communications ( IF 14.7 ) Pub Date : 2018-06-19 , DOI: 10.1038/s41467-018-04709-4
Andrey Aristov , Benoit Lelandais , Elena Rensen , Christophe Zimmer

Single molecule localization microscopy can generate 3D super-resolution images without scanning by leveraging the axial variations of normal or engineered point spread functions (PSF). Successful implementation of these approaches for extended axial ranges remains, however, challenging. We present Zernike Optimized Localization Approach in 3D (ZOLA-3D), an easy-to-use computational and optical solution that achieves optimal resolution over a tunable axial range. We use ZOLA-3D to demonstrate 3D super-resolution imaging of mitochondria, nuclear pores and microtubules in entire nuclei or cells up to ~5 μm deep.

中文翻译:

ZOLA-3D允许在可调的轴向范围内进行灵活的3D定位显微镜。

单分子定位显微镜可以利用正常或工程化点扩散函数(PSF)的轴向变化来生成3D超分辨率图像,而无需扫描。然而,对于扩展的轴向范围,这些方法的成功实施仍然具有挑战性。我们提出了3D的Zernike优化定位方法(ZOLA-3D),这是一种易于使用的计算和光学解决方案,可在可调的轴向范围内实现最佳分辨率。我们使用ZOLA-3D演示了整个核或深至约5μm的细胞中线粒体,核孔和微管的3D超分辨率成像。
更新日期:2018-06-19
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