Abstract In this work, we offered a novel and sensitive electrogenerated chemiluminescence (ECL) biosensing system for detection of p16INK4a gene using the functional paste-like nanofibers composites-modified screen-printed carbon electrode (SPCE). The paste-like nanofibers composites (PG/GR/CS) which were comprised of the electrospun nanofibers (PG, the graphene (GR) doped polycaprolactam 6 (PA6) were prepared via one-step electrospinning), graphene (GR) and chitosan (CS) were served as the nanosized backbones for pyrrole (Py) electropolymerization. The functional paste-like nanofibers composites (PG/GR/CS/PPy) used as a substrate for dsDNA (hybridization reaction of ssDNA1, p16INK4a gene and the Ru(bpy)32+/silver nanoparticles (AgNPs) doped gold (Au) core-shell luminescent composite nanoparticles labeled ssDNA2 (RuAg@AuNPs-ssDNA2)) immobilization. Under optimal conditions, a linear relationship between ECL intensity and p16INK4a gene concentration was found in a range of 0.1 pM–1 nM with the detection limit of 0.05 pM (S/N = 3). This ECL biosensor based on the PG/GR/CS/PPy-modified SPCE demonstrated excellent electrochemical performance for the detection of p16INK4a gene and this platform can be used for the determination of various analytes.

中文翻译：

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基于功能性糊状纳米纤维复合材料修饰的丝网印刷碳电极，一种新型灵敏的电生化学发光生物传感器，用于检测 p16 INK4a 基因

摘要 在这项工作中，我们提供了一种新型且灵敏的电致化学发光 (ECL) 生物传感系统，用于使用功能性糊状纳米纤维复合材料修饰的丝网印刷碳电极 (SPCE) 检测 p16INK4a 基因。糊状纳米纤维复合材料（PG/GR/CS）由静电纺纳米纤维（PG、石墨烯（GR）掺杂的聚己内酰胺6（PA6）通过一步静电纺丝法制备）、石墨烯（GR）和壳聚糖（ CS）用作吡咯（Py）电聚合的纳米级主链。功能性糊状纳米纤维复合材料（PG/GR/CS/PPy）用作 dsDNA（ssDNA1、p16INK4a 基因和 Ru(bpy)32+/银纳米粒子 (AgNPs) 掺杂的金 (Au) 核壳发光复合纳米粒子标记的 ssDNA2 (RuAg@AuNPs-ssDNA2)) 固定。在最佳条件下，ECL 强度与 p16INK4a 基因浓度呈线性关系，范围为 0.1 pM–1 nM，检测限为 0.05 pM (S/N = 3)。这种基于 PG/GR/CS/PPy 修饰的 SPCE 的 ECL 生物传感器在检测 p16INK4a 基因方面表现出优异的电化学性能，该平台可用于测定各种分析物。