Antioxidant activity, neuroprotective properties and bioactive constituents analysis of varying polarity extracts from Eucalyptus globulus leaves,Journal of Food and Drug Analysis

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Antioxidant activity, neuroprotective properties and bioactive constituents analysis of varying polarity extracts from Eucalyptus globulus leaves
Journal of Food and Drug Analysis ( IF 2.6 ) Pub Date : 2018-10-01 , DOI: 10.1016/j.jfda.2018.05.010
Elena González-Burgos ₁ , Mindaugas Liaudanskas _{2,

3} , Jonas Viškelis ₃ , Vaidotas Žvikas ₄ , Valdimaras Janulis ₄ , M Pilar Gómez-Serranillos ₁

Affiliation

Eucalyptus globulus is employed as herbal tea and therapeutical purposes. In this work, it is investigated for first time the neuroprotective activities, based on antioxidant properties, of varying polarity extracts (acetone, ethanol and methanol) from E. globulus leaves and elucidate their main bioactive constituents. Methanol and acetone extracts contained the highest phenolic compounds amount and chlorogenic acid was the major compound identified by UPLC-ESI-MS/MS. Moreover, the three tested extracts showed significant antioxidant properties, varying their potency depending on the in vitro technique used. Furthermore, E.globulus extracts were effective in ameliorating H2O2-induced oxidative stress by increasing cell viability, GSH levels and antioxidant enzymes activity and, by decreasing ROS production and lipid peroxidation levels in SH-SY5Y cells. Taken together, E.globulus leaves extracts could be used as raw material for food and pharmaceutical supplements for their high content in antioxidant compounds with health benefits properties against oxidative stress.

中文翻译：

球状桉叶不同极性提取物的抗氧化活性、神经保护特性和生物活性成分分析

Eucalyptus globulus 用作凉茶和治疗目的。在这项工作中，首次研究了基于抗氧化特性的不同极性提取物（丙酮、乙醇和甲醇）的神经保护活性，并阐明了它们的主要生物活性成分。甲醇和丙酮提取物中酚类化合物含量最高，绿原酸是 UPLC-ESI-MS/MS 鉴定的主要化合物。此外，三种测试的提取物显示出显着的抗氧化特性，其效力取决于所使用的体外技术。此外，E.globulus 提取物通过增加细胞活力、谷胱甘肽水平和抗氧化酶活性来有效改善 H2O2 诱导的氧化应激，并且，通过降低 SH-SY5Y 细胞中的 ROS 产生和脂质过氧化水平。总之，E.globulus 叶子提取物可用作食品和药物补充剂的原料，因为它们含有高含量的抗氧化化合物，具有抗氧化应激的健康益处。

更新日期：2018-10-01

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