Independent Control over Multiple Cell Types in Space and Time Using Orthogonal Blue and Red Light Switchable Cell Interactions,Advanced Science

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Independent Control over Multiple Cell Types in Space and Time Using Orthogonal Blue and Red Light Switchable Cell Interactions
Advanced Science ( IF 14.3 ) Pub Date : 2018-06-17 , DOI: 10.1002/advs.201800446
Simge G Yüz _{1,

2} , Julia Ricken _{1,

2} , Seraphine V Wegner ₁

Affiliation

Independent control over multiple cell–material interactions with high spatiotemporal resolution is a key for many biomedical applications and understanding cell biology, as different cell types can perform different tasks in a multicellular context. In this study, the binding of two different cell types to materials is orthogonally controlled with blue and red light providing independent regulation in space and time. Cells expressing the photoswitchable protein cryptochrome 2 (CRY2) on cell surface bind to N‐truncated CRY‐interacting basic helix–loop–helix protein 1 (CIBN)‐immobilized substrates under blue light and cells expressing the photoswitchable protein phytochrome B (PhyB ) on cell surface bind to phytochrome interaction factor 6 (PIF6)‐immobilized substrates under red light, respectively. These light‐switchable cell interactions provide orthogonal and noninvasive control using two wavelengths of visible light. Moreover, both cell–material interactions are dynamically switched on under light and reversible in the dark. The specificity of the CRY2/CIBN and PhyB/PIF6 interactions and their response to different wavelengths of light allow selectively activating the binding of one cell type with blue and the other cell type with red light in the presence of the other cell type.

中文翻译：

使用正交蓝光和红光可切换细胞相互作用在空间和时间上独立控制多种细胞类型

以高时空分辨率独立控制多种细胞-材料相互作用是许多生物医学应用和理解细胞生物学的关键，因为不同的细胞类型可以在多细胞环境中执行不同的任务。在这项研究中，两种不同细胞类型与材料的结合是通过蓝光和红光正交控制的，提供了空间和时间的独立调节。在蓝光下，细胞表面表达光开关蛋白隐花色素 2 (CRY2) 的细胞与 N-截短的 CRY 相互作用的碱性螺旋-环-螺旋蛋白 1 (CIBN) 固定的底物结合，而细胞表面表达光开关蛋白光敏色素 B (PhyB)在红光下，细胞表面分别与光敏色素相互作用因子 6 (PIF6) 固定的底物结合。这些光可切换的细胞相互作用使用两种波长的可见光提供正交和非侵入性控制。此外，细胞与材料的相互作用在光下动态开启，在黑暗中可逆。CRY2/CIBN 和 PhyB/PIF6 相互作用的特异性及其对不同波长光的响应允许在另一种细胞类型存在的情况下选择性地激活一种细胞类型与蓝光的结合以及另一种细胞类型与红光的结合。

更新日期：2018-06-17

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