Rhinovirus (RV) infections are major triggers of acute exacerbations of severe respiratory diseases such as pre-school wheeze, asthma and chronic obstructive pulmonary disease (COPD). The occurrence of numerous RV types is a major challenge for the identification of the culprit virus types and for the improvement of virus type-specific treatment strategies. Here, we develop a chip containing 130 different micro-arrayed RV proteins and peptides and demonstrate in a cohort of 120 pre-school children, most of whom had been hospitalized due to acute wheeze, that it is possible to determine the culprit RV species with a minute blood sample by serology. Importantly, we identify RV-A and RV-C species as giving rise to most severe respiratory symptoms. Thus, we have generated a chip for the serological identification of RV-induced respiratory illness which should be useful for the rational development of preventive and therapeutic strategies targeting the most important RV types.

中文翻译：

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基于 PreDicta 芯片的鼻病毒引起的喘息的高分辨率诊断。


鼻病毒（RV）感染是严重呼吸道疾病急性发作的主要诱因，例如学前喘息、哮喘和慢性阻塞性肺病（COPD）。多种RV类型的出现对于识别罪魁祸首病毒类型和改进病毒类型特异性治疗策略来说是一个重大挑战。在这里，我们开发了一种包含 130 种不同微阵列 RV 蛋白和肽的芯片，并在 120 名学龄前儿童（其中大多数人因急性喘息而住院）中证明，可以通过以下方法确定罪魁祸首 RV 物种：通过血清学检测获得微量血液样本。重要的是，我们确定 RV-A 和 RV-C 物种会引起最严重的呼吸道症状。因此，我们开发了一种用于 RV 引起的呼吸道疾病血清学鉴定的芯片，这对于合理制定针对最重要的 RV 类型的预防和治疗策略很有用。