To promote drug delivery to exact sites and cell types, the surface of nanocarriers is functionalized with targeting antibodies or ligands, typically coupled by covalent chemistry. Once the nanocarrier is exposed to biological fluid such as plasma, however, its surface is inevitably covered with various biomolecules forming the protein corona, which masks the targeting ability of the nanoparticle. Here, we show that we can use a pre-adsorption process to attach targeting antibodies to the surface of the nanocarrier. Pre-adsorbed antibodies remain functional and are not completely exchanged or covered by the biomolecular corona, whereas coupled antibodies are more affected by this shielding. We conclude that pre-adsorption is potentially a versatile, efficient and rapid method of attaching targeting moieties to the surface of nanocarriers.

为了促进药物递送至确切的部位和细胞类型，通常通过共价化学偶联的靶向抗体或配体对纳米载体的表面进行功能化。然而，一旦纳米载体暴露于诸如血浆的生物流体中，其表面就不可避免地被形成蛋白质电晕的各种生物分子覆盖，这掩盖了纳米粒子的靶向能力。在这里，我们表明我们可以使用预吸附过程将靶向抗体附着到纳米载体的表面。预先吸附的抗体仍保持功能，不会被生物分子电晕完全交换或覆盖，而偶联的抗体则更容易受到这种屏蔽的影响。我们得出的结论是，预吸附可能是一种将靶向部分附着到纳米载体表面的通用，有效和快速的方法。