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Single-file transport of water through membrane channels.
Faraday Discussions ( IF 3.3 ) Pub Date : 2018-06-18 , DOI: 10.1039/c8fd00122g Andreas Horner 1 , Peter Pohl
Faraday Discussions ( IF 3.3 ) Pub Date : 2018-06-18 , DOI: 10.1039/c8fd00122g Andreas Horner 1 , Peter Pohl
Affiliation
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Water at interfaces governs many processes on the molecular scale from electrochemical and enzymatic reactions to protein folding. Here we focus on water transport through proteinaceous pores that are so narrow that the water molecules cannot overtake each other in the pore. After a short introduction into the single-file transport theory, we analyze experiments in which the unitary water permeability, pf, of water channel proteins (aquaporins, AQPs), potassium channels (KcsA), and antibiotics (gramicidin-A derivatives) has been obtained. A short outline of the underlying methods (scanning electrochemical microscopy, fluorescence correlation spectroscopy, measurements of vesicle light scattering) is also provided. We conclude that pf increases exponentially with a decreasing number NH of hydrogen bond donating or accepting residues in the channel wall. The variance in NH is responsible for a more than hundredfold change in pf. The dehydration penalty at the channel mouth has a smaller effect on pf. The intricate link between pf and the Gibbs activation energy barrier, ΔG‡t, for water flow suggests that conformational transitions of water channels act as a third determinant of pf.
中文翻译：
通过膜通道单列传输水。
界面处的水控制着分子尺度上的许多过程，从电化学和酶反应到蛋白质折叠。在这里，我们重点研究水通过蛋白质孔隙的运输，这些孔隙非常狭窄，以至于水分子在孔隙中无法相互超越。在简单介绍单文件传输理论后，我们分析了水通道蛋白（水通道蛋白、AQP）、钾通道（KcsA）和抗生素（短杆菌肽-A衍生物）的单一水渗透性p f的实验。已获得。还提供了基本方法（扫描电化学显微镜、荧光相关光谱、囊泡光散射测量）的简短概述。我们得出结论，随着通道壁中氢键给予或接受残基数量N H的减少， p f呈指数增加。N H的方差导致p f发生超过一百倍的变化。通道口处的脱水损失对p f的影响较小。p f和水流的吉布斯活化能垒 Δ G ‡ t之间的复杂联系表明，水通道的构象转变是p f的第三个决定因素。
更新日期：2018-09-28
中文翻译：
通过膜通道单列传输水。
界面处的水控制着分子尺度上的许多过程，从电化学和酶反应到蛋白质折叠。在这里，我们重点研究水通过蛋白质孔隙的运输，这些孔隙非常狭窄，以至于水分子在孔隙中无法相互超越。在简单介绍单文件传输理论后，我们分析了水通道蛋白（水通道蛋白、AQP）、钾通道（KcsA）和抗生素（短杆菌肽-A衍生物）的单一水渗透性p f的实验。已获得。还提供了基本方法（扫描电化学显微镜、荧光相关光谱、囊泡光散射测量）的简短概述。我们得出结论，随着通道壁中氢键给予或接受残基数量N H的减少， p f呈指数增加。N H的方差导致p f发生超过一百倍的变化。通道口处的脱水损失对p f的影响较小。p f和水流的吉布斯活化能垒 Δ G ‡ t之间的复杂联系表明，水通道的构象转变是p f的第三个决定因素。
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