Bergenin loaded gum xanthan stabilized silver nanoparticles suppress synovial inflammation through modulation of the immune response and oxidative stress in adjuvant induced arthritic rats†,Journal of Materials Chemistry B

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Bergenin loaded gum xanthan stabilized silver nanoparticles suppress synovial inflammation through modulation of the immune response and oxidative stress in adjuvant induced arthritic rats†
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2018-06-16 00:00:00 , DOI: 10.1039/c8tb00672e
Komal Rao _{1,

2,

3,

4,

5} , Talat Roome _{4,

6,

7,

8,

9} , Sabahat Aziz _{4,

6,

7,

8,

9} , Anam Razzak _{4,

6,

7,

8,

9} , Ghulam Abbas _{1,

2,

3,

4,

5} , Muhammad Imran _{1,

2,

3,

4,

5} , Tooba Jabri _{1,

2,

3,

4,

5} , Jasra Gul _{1,

2,

3,

4,

5} , Munawar Hussain _{4,

5,

9,

10} , Bushra Sikandar _{7,

8,

9,

11,

12} , Shaheen Sharafat _{4,

5,

8,

9,

12} , Muhammad Raza Shah _{1,

2,

3,

4,

5}

Affiliation

Bergenin (BG) is a naturally occurring C-glycoside with demonstrated anti-arthritic potential. Its therapeutic efficacy is compromised due to its lower absorption and instability at neutral-basic pH. The present study reports fabrication of gum xanthan (GX) stabilized silver nanoparticles (AgNPs) with BG for anti-arthritic activity in a CFA-induced arthritis model targeting ROS, cytokines and TLR expression. NPs were characterized through UV-vis, zetasizer, FT-IR and AFM. Oral administration of BG loaded NPs (1 mg kg⁻¹) exhibited potent anti-arthritic activity with a minimal arthritic score, mild to moderate paw tissue swelling, reduced degenerative changes along with mild articular changes and less influx of inflammatory cells in macroscopic X-ray and histological examination. Administration of BG and its NPs suppressed the levels of reactive oxygen species (ROS) significantly as compared to the arthritic control group. Moreover, increased production of O₂˙⁻ in human neutrophils, stimulated by opsonized zymosan (OZ) and phorbol-12-myristate-13-acetate (PMA) was also suppressed. BG and its loaded NPs were revealed to antagonize the oxidative stress via interference with the NADPH oxidase metabolic pathway. Their anti-oxidant activity was further assessed by their inhibitory effect against TLR (TRL-2 & -4) and cytokine (IL-1β, IL-6 and TNF-α) production. The current investigation validates GX stabilized AgNPs as stable and promising multi-targeted therapeutic nano-cargo for BG delivery with efficient treatment of RA.

中文翻译：

载有白菜素的胶黄原胶稳定的银纳米颗粒可通过调节佐剂诱发的关节炎大鼠的免疫应答和氧化应激来抑制滑膜炎症†

Bergenin（BG）是一种天然存在的C-糖苷，具有抗关节炎潜力。由于其在中性碱性pH值下的较低吸收和不稳定性，其治疗功效受到了损害。本研究报告了在BFA诱导的针对ROS，细胞因子和TLR表达的CFA诱导的关节炎模型中，具有BG的胶黄原胶（GX）稳定的银纳米颗粒（AgNPs）的制备。通过紫外可见光，zetasizer，FT-IR和AFM对NP进行表征。口服含BG的NP（1 mg kg ^-1）在宏观X射线和组织学检查中显示出有效的抗关节炎活性，具有最小的关节炎评分，足爪中度至中度肿胀，变性变化减少，关节轻度变化以及炎性细胞流入减少。与关节炎对照组相比，BG及其NP的给药显着抑制了活性氧（ROS）的水平。此外，提高了生产的O- ₂ ˙ ^-在人嗜中性粒细胞，刺激通过调理酵母（OZ）和佛波醇-12-肉豆蔻酸酯-13-乙酸酯（PMA）中的溶液也被抑制。揭示BG及其负载的NPs通过拮抗氧化应激。干扰NADPH氧化酶代谢途径。通过对TLR（TRL-2＆-4）和细胞因子（IL-1β，IL-6和TNF-α）的抑制作用进一步评估了它们的抗氧化活性。目前的研究证实了GX稳定的AgNPs是稳定且有前途的多靶点治疗性纳米货物，可有效治疗RA，用于BG递送。

更新日期：2018-06-16

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