Recently, applications of traditional medicine in tissue engineering have gained increasing attention. The components in ginger possess potent antioxidant and anti-inflammatory activities, which are considered to be closely associated with its cancer chemopreventive potential. Here, we report a composite system with a highly efficient, bio-responsive, and potent antioxidant-loaded phosphorylated polymer hydroxyapatite for bone tissue engineering based on the anti-osteosarcoma effect of 6-gingerol. The physicochemical properties of the nanocomposites were characterized by Fourier transform spectroscopy, X-ray diffraction, scanning electron microscopy, and in vitro drug release, and the antioxidant activity was determined by UV-vis spectroscopy. Osteoblast (MG63) adhesion and toxicity were tested against an osteosarcoma cell line (Saos-2). The ALP activities and gene expression after HAP/PCS and HAP/PCS/G nanocomposite treatment were investigated. HAP/PCS/G suppressed osteosarcoma progression. Taken together, HAP/PCS/G could be exploited for advanced graft material development to accelerate bone regeneration as HAP/PCS stimulates osteogenesis and 6-gingerol suppresses osteosarcoma progression.

中文翻译：

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磷酸化的壳聚糖武装羟基磷灰石纳米复合材料，可促进对成骨细胞和骨肉瘤细胞的活性†

近来，传统医学在组织工程中的应用越来越受到关注。姜中的成分具有有效的抗氧化剂和抗炎活性，被认为与其抗癌化学作用密切相关。在这里，我们报告了一种基于6-姜油素的抗骨肉瘤作用的，具有高效，生物响应性和强效抗氧化剂负载的磷酸化聚合物羟基磷灰石的复合系统，用于骨组织工程。纳米复合材料的理化性质通过傅里叶变换光谱，X射线衍射，扫描电子显微镜和体外进行了表征药物释放，并通过紫外可见光谱法测定抗氧化活性。测试了成骨细胞（MG63）对骨肉瘤细胞系（Saos-2）的粘附性和毒性。研究了HAP / PCS和HAP / PCS / G纳米复合材料处理后ALP活性和基因表达。HAP / PCS / G抑制骨肉瘤进展。两者合计，HAP / PCS / G可以用于先进的移植材料开发，以加速骨再生，因为HAP / PCS可以刺激成骨作用，而6-姜油醇可以抑制骨肉瘤的进展。