AbstractA non-fouling electrochemical immunosensor is described for determination of the tumor biomarker carcinoembryonic antigen (CEA). It is based on the use of composite wires made by chemical grafting of hyaluronic acid onto polyaniline nanowires. The modified nanowires possess excellent antifouling property both in single protein solutions and in dilute serum samples. The current of immunoelectrode exhibits a linear response in the 0.01 pg mL−1 to 10,000 pg mL−1 CEA concentration range and 0.0075 pg mL−1 detection limit. This work demonstrates that coating an electrode with hyaluronic acid can largely reduce unspecific adsorption of proteins on the electrode surface. Graphical abstractSchematic of a nonfouling electrochemical immunosensor for the carcinoembryonic antigen. It is based on novel composite wires made through the chemical grafting of easily available hyaluronic acid (HA) onto polyaniline (PANI) nanowires. The HA/PANI demonstrated excellent antifouling property both in single protein solutions and human serum samples.

中文翻译：

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基于透明质酸包裹聚苯胺纳米线的癌胚抗原无污染伏安免疫传感器

摘要描述了一种用于测定肿瘤生物标志物癌胚抗原 (CEA) 的无污染电化学免疫传感器。它基于使用通过将透明质酸化学接枝到聚苯胺纳米线上而制成的复合线。改性纳米线在单一蛋白质溶液和稀释血清样品中均具有优异的防污性能。免疫电极的电流在 0.01 pg mL-1 至 10,000 pg mL-1 CEA 浓度范围和 0.0075 pg mL-1 检测限内表现出线性响应。这项工作表明，用透明质酸涂覆电极可以大大减少电极表面上蛋白质的非特异性吸附。用于癌胚抗原的无污染电化学免疫传感器的图形摘要示意图。它基于通过将容易获得的透明质酸 (HA) 化学接枝到聚苯胺 (PANI) 纳米线上而制成的新型复合线。HA/PANI 在单一蛋白质溶液和人血清样品中均表现出优异的防污性能。