Mimicking subcellular compartments containing enzymes in organisms is considered a promising approach to substitute for missing or lost cellular functions. Inspired by the multicompartment structures of cellular architectures, we present a novel multienzyme system based on hollow hydrogel microcapsules with flexible enzymatic inverse opal particles. Benefiting from the precise operation capability of the microfluidic electrospray and the remarkable structural color marks in the inverse opal particles, we developed a multienzyme system with controllable number, type, and spatial arrangement of the encapsulated enzymes. The hydrogel shells also could improve enzyme stability against proteolysis in the system. The multienzyme system containing alcohol oxidase and catalase could act as a cascade biocatalyst and reduce alcohol levels in media, providing an alternative antidote and prophylactic for alcohol intoxication. These features indicated that our strategy provides an ideal enzyme cascade reaction system for complex biocatalysis and biomimetic functions of some organelles or organs.

中文翻译：

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微流电喷雾微胶囊中的仿生酶级联反应系统。

在生物体中模仿包含酶的亚细胞区室被认为是替代丢失或丧失细胞功能的有前途的方法。受细胞结构的多隔室结构的启发，我们提出了一种基于中空水凝胶微胶囊和柔性酶促反蛋白石颗粒的新型多酶系统。得益于微流电喷雾的精确操作能力和蛋白石反面颗粒中显着的结构颜色标记，我们开发了一种多酶系统，其包封酶的数量，类型和空间排列可控。水凝胶壳还可以提高酶对系统中蛋白水解的稳定性。含有酒精氧化酶和过氧化氢酶的多酶系统可以充当级联生物催化剂，并降低培养基中的酒精含量，提供酒精中毒的替代解毒剂和预防剂。这些特征表明，我们的策略为某些细胞器或器官的复杂生物催化和仿生功能提供了理想的酶联反应体系。