The treatment of some inborn metabolism errors requires cholesterol substitution therapy. Cholesterol plays a vital role in the human body. Therefore, the majority of cholesterol determination techniques are targeted to blood and blood serum. Nevertheless, cholesterol determination in food is important as well. In this paper, cholesterol determination using differential pulse voltammetry (DPV) in dairy products (e.g., milk, clotted cream, yogurt, butter, etc.) is reported with a novel nonenzymatic sensor based on diphosphonic acid of 1,4-diacetylglycoluril (DPADGU) as an electrode surface modifier. Stable anodic response was obtained from cholesterol on the modified carbon-based electrode. The sensor has high stability, sensitivity (20 μA mol L⁻¹ cm⁻²), and a wide linear range from 1 up to 200 μM. The LOD and LOQ values are 1.5 and 5.1 μM, respectively. The developed methods were successfully applied to the above mentioned dairy products.

中文翻译：

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电化学非酶传感器测定食品中的胆固醇

一些先天性代谢错误的治疗需要胆固醇替代疗法。胆固醇在人体中起着至关重要的作用。因此，大多数胆固醇测定技术都针对血液和血清。尽管如此，食品中胆固醇的测定也很重要。在本文中，报道了一种基于差动脉冲伏安法（DPV）的乳制品（例如牛奶，凝结的奶油，酸奶，黄油等）中胆固醇的测定方法，该方法基于一种新型的基于1,4-二乙酰基甘氨脲的二膦酸的非酶传感器（DPADGU） ）作为电极表面改性剂。从修饰的碳基电极上的胆固醇获得稳定的阳极响应。传感器具有高稳定性，灵敏度（20μAmol L ^-1  cm ^-2），以及从1到200μM的宽线性范围。LOD和LOQ值分别为1.5和5.1μM。所开发的方法已成功应用于上述乳制品。