A generalized approach for NMR studies of lipid–protein interactions based on sparse fluorination of acyl chains†,Chemical Communications

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A generalized approach for NMR studies of lipid–protein interactions based on sparse fluorination of acyl chains†
Chemical Communications ( IF 4.3 ) Pub Date : 2018-06-15 00:00:00 , DOI: 10.1039/c8cc02483a
Alfredo De Biasio _{1,

2,

3} , Alain Ibáñez de Opakua _{1,

2,

3} , Mark J. Bostock _{4,

5,

6,

7} , Daniel Nietlispach _{4,

5,

6,

7} , Tammo Diercks _{1,

2,

3} , Francisco J. Blanco _{1,

2,

3,

8,

9}

Affiliation

Sparse lipid fluorination enhances the lipids' ¹H signal dispersion, enables clean molecular distinction by ¹⁹F NMR, and evinces micelle insertion of proteins via fluorine-induced signal shifts. We present a minimal fluorination scheme, and illustrate the concept on di-(4-fluoro)-heptanoylphosphatidylcholine micelles and solubilised seven-helix transmembrane pSRII protein.

中文翻译：

基于酰基链稀疏氟化的脂质-蛋白质相互作用的NMR研究的通用方法†

稀疏的脂质氟化作用可增强脂质的¹ H信号分散性，并通过¹⁹ F NMR进行清晰的分子区分，并通过氟诱导的信号移位来证明蛋白质的胶束插入。我们提出了一个最小的氟化方案，并说明了对二（4-氟）-庚酰基磷脂酰胆碱胶束和可溶的七螺旋跨膜pSRII蛋白的概念。

更新日期：2018-06-15

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