Vascular grafts for free flap transfers should be of very small diameter and remain patent for approximately three weeks to supply blood until the revascularization from the surrounding tissue is established, with the autologous vein grafts acting as the gold standard. Artificial submillimeter-diameter vascular grafts with clinically useful size of 0.6 mm inner diameter and 5 cm length were prepared and evaluated by replacing the axial artery of free flap in rats. The rat tail artery, selected as a novel bioscaffold material, was decellularized using ultrahigh-hydrostatic pressure (UHP) method and compared with the detergent-based conventional method. To induce rapid endothelialization, the graft lumen was modified with synthesized peptides, having high affinity to the endothelial progenitor cells. The UHP method and peptide modification at 37 °C were found to preserve the extracellular matrix structure well, leading to the stable immobilization of the peptide at the luminal surface. These grafts showed the neointima formation, even at the center position far from the native vessels, remained patent for three weeks, and resulted in the flap survival in the rat free-flap model. The tissue-engineered vascular grafts with functionalized lumen have great future potential as an alternative to autologous vein grafts in free flap transfers.

用于游离皮瓣转移的血管移植物应具有很小的直径，并保持专利权大约三周以供应血液，直到建立周围组织的血运重建为止，而自体静脉移植物是金标准。制备了临床上有用的内径为0.6毫米，长度为5厘米的亚毫米直径人造血管，并通过替换了大鼠游离皮瓣的轴向动脉对其进行了评估。使用超高静水压（UHP）方法将被选为新型生物支架材料的大鼠尾动脉脱细胞，并与基于去污剂的常规方法进行比较。为了诱导快速内皮化，用合成的肽修饰移植物内腔，所述肽对内皮祖细胞具有高亲和力。发现UHP方法和在37°C下对肽进行修饰可以很好地保留细胞外基质结构，从而将肽稳定地固定在腔表面。这些移植物显示出新内膜的形成，即使在远离天然血管的中心位置，也保持了专利权三周，并在大鼠游离皮瓣模型中导致了皮瓣的存活。具有功能性内腔的组织工程化血管移植物在自由皮瓣转移中作为自体静脉移植物的替代物具有巨大的未来潜力。并导致大鼠游离皮瓣模型的皮瓣存活。具有功能性内腔的组织工程化血管移植物在自由皮瓣转移中作为自体静脉移植物的替代品具有巨大的未来潜力。并导致大鼠游离皮瓣模型的皮瓣存活。具有功能性内腔的组织工程化血管移植物在自由皮瓣转移中作为自体静脉移植物的替代物具有巨大的未来潜力。