Natural anthocyanins are safer and nutritious as compared to synthetic pigments; however, their stability is poor. They can produce spontaneous copigmentation with organic acids, leading to the improvement of colour stability, albeit slowly. Box–Behnken experimental design was used to elucidate the mechanism of copigmentation between Vitis amurensis Rupr anthocyanins (0.1 mg/mL) and organic acids (0.87 mg/mL, ferulic acid:d-gluconic acid:caffeic acid:vanillic acid = 1.5:2.5:2.5:0.5, w/w/w/w) promoted by high hydrostatic pressure (HHP; 300 MPa, 2 min). The copigmentation effect and antioxidant activity of anthocyanins were also evaluated. The structure of anthocyanins was analysed using ultraviolet–visible spectroscopy, Fourier-transform infrared spectroscopy, high-performance liquid chromatography, and mass spectrometry. The results of HHP copigmentation showed that the following anthocyanins were newly formed—delphinidin-3-O-catechol, petunidin-3-O-catechol, delphinidin-4-vinyl-catechol, petunidin-3-O-guaiacol, malvidin-4-vinyl-guaiacol, cyanidin-3-O-(6″-O-caffeoyl)-glucoside, peonidin-3-O-(6″-O-caffeoyl)-glucoside, delphinidin-3-O-(6″-O-caffeoyl)-glucoside, malvidin-3-O-glucoside-4-vinyl-guaiacol, and malvidin-3-O-(6″-O-feruloyl)-glucoside—owing to appropriate modifications that increased the copigmentation rate (R = 42.12%), photo-thermal stability (R > 45%), and potential antioxidant activities expressed in vivo (p < 0.01 vs. Model Group).

与合成颜料相比，天然花青素更安全和营养。但是，它们的稳定性差。它们可以产生自发的有机酸色素沉着，尽管缓慢，但可以改善色彩稳定性。使用Box–Behnken实验设计阐明了葡萄（Vitis amurensis）Rupr花青素（0.1 mg / mL）和有机酸（0.87 mg / mL，阿魏酸：d-葡萄糖酸：咖啡酸：香草酸= 1.5：2.5：2.5：0.5，w / w / w / w）由高静水压（HHP; 300 MPa，2分钟）促进。还评估了花青素的色素沉着效果和抗氧化活性。使用紫外可见光谱，傅立叶变换红外光谱，高效液相色谱和质谱分析了花色苷的结构。HHP copigmentation的结果表明，以下的花青素是新形成的，花翠素-3- ø -catechol，矮牵牛-3- ø -catechol，翠雀素4-乙烯基儿茶酚，矮牵牛-3- ø -guaiacol，二甲花翠素-4-乙烯基愈创木酚，花青素3- O-（6″ -O-咖啡酰）-葡萄糖苷，peonidin-3- O-（6″-O-咖啡酰）-葡糖苷，delphinidin-3- O-（6″ -O- Caffeoyl）-葡糖苷，malvidin-3- O-葡糖苷-4-乙烯基-愈创木酚和Malvidin-3- O-（6″ -O -阿魏酰基）-葡萄糖苷-由于进行了适当的修饰，从而提高了色素沉着率（R = 42.12％），光热稳定性（R> 45％）和体内潜在的抗氧化活性（相对于模型组，p  <0.01 ）。