The commercial STREAMLINE Direct HST adsorbent was employed to investigate the adsorption characteristics of lysozyme in complex chicken egg white (CEW). The effects of operating parameters, including adsorption pH, ionic strength, and hydrophobicity of liquids on binding capacity, were examined using a well-mixed contactor. To determine the elution strategy, the experiments were carried out in a small packed bed. The lysozyme was completely eluted by 0.5 M NaCl in 40 mM carbonate buffer (pH 12) at a high liquid velocity of 200 cm/h. The effect of rotating speed on fluidization characteristics was further investigated by using a stirred fluidized bed process. Smoother fluidization was observed when the rotating speed reached 200 rpm and lysozyme was directly recovered from highly viscous CEW in a single step with a high yield of 94.3% and a purification factor of 15.7.

利用商用STREAMLINE Direct HST吸附剂研究溶菌酶在复杂鸡蛋白（CEW）中的吸附特性。使用良好混合的接触器检查了操作参数的影响，包括吸附pH，离子强度和液体的疏水性对结合能力的影响。为了确定洗脱策略，在小型填充床中进行了实验。用40 mM碳酸盐缓冲液（pH 12）中的0.5 M NaCl在200 cm / h的高液体速度下完全溶解了溶菌酶。通过使用搅拌流化床工艺进一步研究了旋转速度对流化特性的影响。当转速达到200 rpm时可以观察到更流畅的流态化，并且可以单步从高粘度CEW中直接回收溶菌酶，产率高达94。