Target‐Induced Catalytic Assembly of Y‐Shaped DNA and Its Application for In Situ Imaging of MicroRNAs,Angewandte Chemie International Edition

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Target‐Induced Catalytic Assembly of Y‐Shaped DNA and Its Application for In Situ Imaging of MicroRNAs
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2018-07-13 , DOI: 10.1002/anie.201804741
Chang Xue ₁ , Shu-Xin Zhang ₁ , Chang-He Ouyang ₁ , Dingran Chang ₂ , Bruno J. Salena ₃ , Yingfu Li ₂ , Zai-Sheng Wu ₁

Affiliation

DNA is a highly programmable material that can be configured into unique high‐order structures, such as DNA branched junctions containing multiple helical arms converging at a center. Herein we show that DNA programmability can deliver in situ growth of a 3‐way junction‐based DNA structure (denoted Y‐shaped DNA) with the use of three hairpin‐shaped DNA molecules as precursors, a specific microRNA target as a recyclable trigger, and a DNA polymerase as a driver. We demonstrate that the Y‐shaped configuration comes with the benefit of restricted freedom of movement in confined cellular environment, which makes the approach ideally suited for in situ imaging of small RNA targets, such as microRNAs. Comparative analysis illustrates that the proposed imaging technique is superior to both the classic fluorescence in situ hybridization (FISH) method and an analogous amplified imaging method via programmed growth of a double‐stranded DNA (rather than Y‐shaped DNA) product.

中文翻译：

目标诱导的Y型DNA的催化组装及其在MicroRNA原位成像中的应用

DNA是一种高度可编程的材料，可以配置成独特的高级结构，例如包含多个螺旋臂汇聚在一个中心的DNA分支结。本文显示，利用三个发夹形DNA分子作为前体，特定的microRNA靶作为可回收的触发物，DNA的可编程性可以实现基于三向连接的DNA结构（称为Y形DNA）的原位生长，和DNA聚合酶作为驱动因子。我们证明了Y形配置的好处是在受限的细胞环境中移动受限，这使该方法非常适合于小RNA靶标（如microRNA）的原位成像。

更新日期：2018-07-13

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