Prostate cancer is prevalent among cancers in men. A simple method for screening of reliable biomarkers is pivotal for early detection of prostate cancer. Prostate-specific antigen (PSA) has been a commonly used biomarker for prostate cancer, in spite of its false-positive limitation. On the other hand, alpha-methylacyl-CoA racemase (AMACR), a metabolic enzyme, has been proven to be a highly expressed biomarker in prostate cancer cells. Therefore, a method or tool, which can detect either PSA or AMACR or both simply, cost effectively, and with high sensitivity and selectivity is desirable. We describe a novel bioconjugated, single-use biosensor capable of detecting both PSA and AMACR antigens in undiluted human serum. The preparation of the biosensor by the bioconjugation mechanism occurred within a day, which could be completed prior to actual testing. The effectiveness of the bioconjugation mechanism and the coverage of the electrode surface of the biosensor were experimentally assessed. Measurements of PSA and AMACR antigens and the specificity of the biosensor were carried out using differential pulse voltammetry. This biosensor was single-use and cost-effective and required a small quantity of test medium and relatively short preparation time, providing a very attractive biosensor for the detection of the biomarkers of prostate cancer.

中文翻译：

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生物共轭的一次性生物传感器，用于检测前列腺癌的生物标志物

前列腺癌在男性癌症中普遍存在。筛选可靠生物标志物的简单方法对于早期检测前列腺癌至关重要。尽管前列腺特异性抗原（PSA）具有假阳性限制，但它一直是前列腺癌的常用生物标志物。另一方面，已证明α-甲基酰基辅酶A消旋酶（AMACR）是一种代谢酶，在前列腺癌细胞中是高度表达的生物标志物。因此，需要一种能够成本有效且具有高灵敏度和选择性的，可以简单地检测PSA或AMACR或两者的方法或工具。我们描述了一种新型的生物共轭的一次性生物传感器，能够检测未稀释的人血清中的PSA和AMACR抗原。通过生物共轭机制制备生物传感器的过程一天之内就完成了，可以在实际测试之前完成。实验评估了生物共轭机制的有效性和生物传感器电极表面的覆盖范围。使用差动脉冲伏安法测量PSA和AMACR抗原以及生物传感器的特异性。这种生物传感器是一次性的且具有成本效益，并且需要少量的测试介质和相对较短的制备时间，为检测前列腺癌的生物标志物提供了非常有吸引力的生物传感器。