Endogenous exosome labelling with an amphiphilic NIR-fluorescent probe†,Chemical Communications

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Endogenous exosome labelling with an amphiphilic NIR-fluorescent probe†
Chemical Communications ( IF 4.3 ) Pub Date : 2018-06-14 00:00:00 , DOI: 10.1039/c8cc02135j
Marco P. Monopoli _{1,

2,

3,

4} , Andrea Zendrini _{5,

6,

7,

8} , Dan Wu _{1,

2,

3,

4} , Shane Cheung _{1,

2,

3,

4} , Gonzalo Sampedro _{1,

2,

3,

4} , Brendan Ffrench _{2,

3,

4,

9} , John Nolan _{2,

3,

4,

10} , Olga Piskareva _{2,

3,

4,

10} , Raymond L. Stalings _{2,

3,

4,

10} , Serena Ducoli _{5,

6,

7,

8} , Paolo Bergese _{5,

6,

7,

8} , Donal F. O’Shea _{1,

2,

3,

4}

Affiliation

The recognition of the biological, diagnostic and medical importance of exosomes has given rise to an urgent need for efficient labelling of these extracellular vesicles in ways that do not alter their inherent characteristics. We report for the first time an endogenous method to NIR-fluorescent labelled exosomes using an amphiphilic probe without the need for immunolabelling or synthetic or chromatographic manipulation of exosomes. Comparative analyses of labelled and unlabelled exosomes with NTA, AFM, flow cytometry and immunoblot analysis all show a high degree of similarity. Spectroscopic analysis and fluorescence imaging confirmed the ability to visualise purified NIR-exosomes.

中文翻译：

两亲NIR荧光探针标记内源性外泌体†

认识到外泌体的生物学，诊断和医学重要性，迫切需要以不改变其固有特性的方式对这些细胞外囊泡进行有效标记。我们首次报告了使用两亲性探针对NIR荧光标记的外泌体进行内源性检测的方法，无需对外泌体进行免疫标记或合成或色谱处理。用NTA，AFM，流式细胞仪和免疫印迹分析对标记和未标记的外泌体进行的比较分析均显示出高度的相似性。光谱分析和荧光成像证实了可视化纯化的NIR外泌体的能力。

更新日期：2018-06-14

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