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Indirect Electrochemical Detection of NADH Through an Active Stainless Steel Fiber Felt (SSFF) Electrode Decorated With the Amino‐Graphene/Nafion Nano Composite Films
ChemistrySelect ( IF 1.9 ) Pub Date : 2018-06-13 , DOI: 10.1002/slct.201800743 Wenqiong Su 1 , Zonglin Li 1 , Shuopeng Liu 1 , Xianting Ding 1
ChemistrySelect ( IF 1.9 ) Pub Date : 2018-06-13 , DOI: 10.1002/slct.201800743 Wenqiong Su 1 , Zonglin Li 1 , Shuopeng Liu 1 , Xianting Ding 1
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As the energy transfer mediator in the metabolism of human beings, dihydronicotinamide adenine dinucleotide (NADH) is considered as an important biomarker, the concentration of which is correlated to several lethal diseases, such as breast cancer and Parkinson's disease. Due to the high oxidation potential encountered for NADH direct oxidation, herein, we report an active electrode for indirect electrochemical detection of NADH at low potential with the inexpensive stainless steel fiber felt (SSFF). The SSFF electrodes were modified through the layer‐by‐layer assembling of amino‐graphene (AG) fragments and Nafion, and characterized by cyclic voltammetry (CV), differential pulse voltammetry (DPV), scanning electron microscope (SEM) and X‐ray photoelectron spectroscopy (XPS). Taking the peak current at the apparent oxidation potential (‐0.2 V) as the sensing signal, the proposed SSFF active electrode presented a stable logarithmic detection range from 100 nM to 300 μM with R2=0.996. The regeneration process was also explored and proved to be robust. Finally, the SSFF active electrode was applied to sense NADH in a bio‐mixture solution prepared from tumor cell lysate. The experimental results demonstrated that, with a facile processing, the corrosion resistant and low cost SSFF electrodes could serve as a reliable and practical probe for NADH detection, which would further benefit the clinical diagnostics of NADH‐related diseases.
中文翻译：
通过用氨基石墨烯/ Nafion纳米复合膜修饰的不锈钢纤维毡（SSFF）电极间接检测NADH
作为人类代谢中的能量转移介质，二氢烟碱酰胺腺嘌呤二核苷酸（NADH）被认为是重要的生物标志物，其浓度与几种致死性疾病（例如乳腺癌和帕金森氏病）相关。由于NADH直接氧化遇到的高氧化电位，因此，本文报道了一种活性电极，可通过廉价的不锈钢纤维毡（SSFF）在低电位间接电化学检测NADH。SSFF电极通过氨基石墨烯（AG）片段和Nafion的逐层组装进行了修饰，并通过循环伏安法（CV），差动脉冲伏安法（DPV），扫描电子显微镜（SEM）和X射线进行了表征光电子能谱（XPS）。以表观氧化电位（-0。2 = 0.996。还研究了再生过程，并证明了再生过程的鲁棒性。最后，将SSFF活性电极应用于从肿瘤细胞裂解液制备的生物混合物溶液中检测NADH。实验结果表明，通过简便的工艺，耐腐蚀，低成本的SSFF电极可以作为NADH检测的可靠实用探针，这将进一步有益于NADH相关疾病的临床诊断。
更新日期：2018-06-13
中文翻译：
通过用氨基石墨烯/ Nafion纳米复合膜修饰的不锈钢纤维毡（SSFF）电极间接检测NADH
作为人类代谢中的能量转移介质，二氢烟碱酰胺腺嘌呤二核苷酸（NADH）被认为是重要的生物标志物，其浓度与几种致死性疾病（例如乳腺癌和帕金森氏病）相关。由于NADH直接氧化遇到的高氧化电位，因此，本文报道了一种活性电极，可通过廉价的不锈钢纤维毡（SSFF）在低电位间接电化学检测NADH。SSFF电极通过氨基石墨烯（AG）片段和Nafion的逐层组装进行了修饰，并通过循环伏安法（CV），差动脉冲伏安法（DPV），扫描电子显微镜（SEM）和X射线进行了表征光电子能谱（XPS）。以表观氧化电位（-0。2 = 0.996。还研究了再生过程，并证明了再生过程的鲁棒性。最后，将SSFF活性电极应用于从肿瘤细胞裂解液制备的生物混合物溶液中检测NADH。实验结果表明，通过简便的工艺，耐腐蚀，低成本的SSFF电极可以作为NADH检测的可靠实用探针，这将进一步有益于NADH相关疾病的临床诊断。
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