Recent Advances in Engineering the Stem Cell Microniche in 3D,Advanced Science

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Recent Advances in Engineering the Stem Cell Microniche in 3D
Advanced Science ( IF 14.3 ) Pub Date : 2018-06-13 , DOI: 10.1002/advs.201800448
Min Bao ₁ , Jing Xie ₁ , Wilhelm T S Huck ₁

Affiliation

Conventional 2D cell culture techniques have provided fundamental insights into key biochemical and biophysical mechanisms responsible for various cellular behaviors, such as cell adhesion, spreading, division, proliferation, and differentiation. However, 2D culture in vitro does not fully capture the physical and chemical properties of the native microenvironment. There is a growing body of research that suggests that cells cultured on 2D substrates differ greatly from those grown in vivo. This article focuses on recent progress in using bioinspired 3D matrices that recapitulate as many aspects of the natural extracellular matrix as possible. A range of techniques for the engineering of 3D microenvironment with precisely controlled biophysical and chemical properties, and the impact of these environments on cellular behavior, is reviewed. Finally, an outlook on future challenges for engineering the 3D microenvironment and how such approaches would further our understanding of the influence of the microenvironment on cell function is provided.

中文翻译：

3D 干细胞微生态工程的最新进展

传统的二维细胞培养技术为了解细胞粘附、扩散、分裂、增殖和分化等各种细胞行为的关键生化和生物物理机制提供了基础见解。然而，体外二维培养并不能完全捕捉原生微环境的物理和化学特性。越来越多的研究表明，在 2D 基质上培养的细胞与体内生长的细胞有很大不同。本文重点介绍使用仿生 3D 矩阵的最新进展，该矩阵尽可能概括了天然细胞外基质的许多方面。综述了一系列具有精确控制的生物物理和化学特性的 3D 微环境工程技术，以及这些环境对细胞行为的影响。最后，展望了 3D 微环境工程的未来挑战，以及这些方法如何进一步加深我们对微环境对细胞功能影响的理解。

更新日期：2018-06-13

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