Coaxial electrospray has been used effectively for several dual-emitter applications, but has not been utilized for the study of rapid in-source chemistry. In this paper, we report the fabrication of a coaxial, micro-volume dual-emitter through the modification of a manufacturer’s standard electrospray probe. This modification creates rapid mixing inside the Taylor cone and the ability to manipulate fast reactions using a variety of solvents and analytes. We demonstrate its potential as a low-cost, dual-emitter assembly for diverse applications through three examples: relative ionization in a biphasic electrospray, hydrogen-deuterium exchange, and protein supercharging.

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同轴电喷雾已有效地用于多种双发射器应用，但尚未用于快速源化学的研究。在本文中，我们报告了通过修改制造商的标准电喷雾探针来制造同轴，微体积双发射器的过程。这种修改可在泰勒锥内部产生快速混合，并具有使用多种溶剂和分析物进行快速反应的能力。我们通过三个例子证明了其作为低成本，双发射器组件在各种应用中的潜力：双相电喷雾中的相对电离，氢-氘交换和蛋白质增压。

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