A ratiometric fluorescent composite nanomaterial for RNA detection based on graphene quantum dots and molecular probes†,Journal of Materials Chemistry B

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A ratiometric fluorescent composite nanomaterial for RNA detection based on graphene quantum dots and molecular probes†
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2018-06-13 00:00:00 , DOI: 10.1039/c8tb01125g
Guanghan Li _{1,

2,

3,

4,

5} , Yong Liu _{1,

2,

3,

4,

5} , Jie Niu _{1,

2,

3,

4,

5} , Meishan Pei _{1,

2,

3,

4,

5} , Weiying Lin _{1,

2,

3,

4,

5}

Affiliation

RNA plays a central role in controlling cellular functions. Research of the content and distribution of RNA in living cells is of great significance to both biochemistry and biomedicine. However, ratiometric fluorescent probes for the detection of RNA in the cytoplasm and nucleoli are still rarely reported. We herein present the first example of a novel ratiometric fluorescent composite nanomaterial by using graphene quantum dots (GQDs) and a fluorescent probe molecule for the sensitive and selective detection of RNA. HVC-6 was selected as the detection group. The fluorescence was excited at 365 nm and the fluorescence emission at 470 and 610 nm increased gradually with the addition of RNA. The fluorescence intensity ratio of I₆₁₀/I₄₇₀ displayed a linear response to RNA. Furthermore, the developed nanomaterial HVC-6@GQDs showed potential for utilization as a fluorescent RNA probe in living cells.

中文翻译：

一种基于石墨烯量子点和分子探针的用于RNA检测的比例荧光复合纳米材料†

RNA在控制细胞功能中起着核心作用。研究RNA在活细胞中的含量和分布对生物化学和生物医学都具有重要意义。但是，仍然很少报道用于检测细胞质和核仁中RNA的比例荧光探针。我们在此通过使用石墨烯量子点（GQD）和荧光探针分子对RNA进行灵敏和选择性检测，提出了一种新型比例荧光复合纳米材料的第一个例子。选择HVC-6作为检测组。荧光在365 nm处被激发，并且随着RNA的加入，在470和610 nm处的荧光发射逐渐增加。I ₆₁₀ / I ₄₇₀的荧光强度比对RNA表现出线性反应。此外，已开发的纳米材料HVC-6 @ GQDs具有在活细胞中用作荧光RNA探针的潜力。

更新日期：2018-06-13

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