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Facile Immobilization of Enzyme via Co-Electrospinning: A Simple Method for Enhancing Enzyme Reusability and Monitoring an Activity-Based Organic Semiconductor
ACS Omega ( IF 3.7 ) Pub Date : 2018-06-13 00:00:00 , DOI: 10.1021/acsomega.8b00366 Musab M. Aldhahri 1 , Yaaser Q. Almulaiky 2 , Reda M. El-Shishtawy 3 , Waleed Al-Shawafi , Ahmed Alngadh 4 , Rayan Maghrabi
ACS Omega ( IF 3.7 ) Pub Date : 2018-06-13 00:00:00 , DOI: 10.1021/acsomega.8b00366 Musab M. Aldhahri 1 , Yaaser Q. Almulaiky 2 , Reda M. El-Shishtawy 3 , Waleed Al-Shawafi , Ahmed Alngadh 4 , Rayan Maghrabi
Affiliation
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The stability, reusability, and monitoring of enzyme activity have been investigated to improve their efficiency for successful utilization in a broad range of industrial and medical applications. Herein, we present a simple method for fabricating an electrospun fiber/enzyme scaffold via co-electrospinning. The characterization of soluble and immobilized α-amylases with regard to pH, thermal stability, and reusability were studied. An organic light emitting material tris(8-hydroxyquinoline)aluminum was incorporated to monitor the enzyme activity for several reuses.
中文翻译：
通过Co-Electrospinning轻松固定酶：增强酶的可重用性和监测基于活性的有机半导体的简单方法
已经研究了稳定性，可重复使用性和酶活性监测，以提高其在广泛的工业和医学应用中成功利用的效率。在这里，我们提出了一种通过共电纺丝制造电纺纤维/酶支架的简单方法。研究了关于pH，热稳定性和可重复使用性的可溶性和固定化α-淀粉酶的特性。掺入有机发光材料三（8-羟基喹啉）铝以监测酶活性多次重复使用。
更新日期：2018-06-13
中文翻译：
通过Co-Electrospinning轻松固定酶：增强酶的可重用性和监测基于活性的有机半导体的简单方法
已经研究了稳定性，可重复使用性和酶活性监测，以提高其在广泛的工业和医学应用中成功利用的效率。在这里，我们提出了一种通过共电纺丝制造电纺纤维/酶支架的简单方法。研究了关于pH，热稳定性和可重复使用性的可溶性和固定化α-淀粉酶的特性。掺入有机发光材料三（8-羟基喹啉）铝以监测酶活性多次重复使用。
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