This study presents a viable green synthesis approach to produce Ag and Cu nanoparticles (MNPs) by using carbohydrates such as glucose, fructose, sucrose, and starch as reducing agents and describes their antimicrobial activities against Escherichia coli DH5α. Optical and diffused light scattering analyses showed the Ag NPs ranged from 20 to 75 nm and the Cu NPs varied from 20 to 160 nm, which supports the differences in their absorption bands (400–434 nm for Ag and 458–641 nm for Cu). The reducing sugars interacted differently with Ag⁺ and Cu²⁺ based on their size and hydrolysis by NaOH resulting in effective stabilization of Ag⁰ and Cu⁰ and variation in the bactericidal activities of the MNPs. The antibacterial effects of the MNPs were evaluated by measuring the inhibition zones using E. coli DH5α as a test organism. No growth was observed by restreaking different parts of the clearly inhibited zones into new culture plates indicating the bactericidal efficacy of the Ag and Cu MNPs. The Ag NPs were found to be more effective in terms of the size of their inhibition zones (1.21–1.82 cm) compared with those of the Cu NPs (0.0–1.2 cm). This study provides a promising basis for the formulation of a new generation of bactericidal agents.

这项研究提出了一种可行的绿色合成方法，该方法通过使用诸如葡萄糖，果糖，蔗糖和淀粉等碳水化合物作为还原剂来生产Ag和Cu纳米颗粒（MNP），并描述了它们对大肠杆菌DH5α的抗菌活性。光学和漫射光散射分析表明，Ag NP的范围为20至75 nm，Cu NP的范围为20至160 nm，这支持了其吸收谱带的差异（Ag为400–434 nm，Cu为458–641 nm） 。还原糖的大小和被NaOH水解后，它们与Ag ⁺和Cu ²⁺的相互作用不同，从而有效地稳定了Ag ⁰和Cu ⁰和MNPs杀菌活性的变化。通过使用大肠杆菌DH5α作为测试生物来测量抑制区域来评估MNP的抗菌作用。通过将明显抑制的区域的不同部分重新添加到新的培养皿中未观察到生长，表明Ag和Cu MNP的杀菌效力。与铜纳米颗粒（0.0-1.2 cm）相比，银纳米颗粒的抑制区（1.21-1.82 cm）更有效。该研究为新一代杀菌剂的配制提供了有希望的基础。