Label strategies are the mainstream techniques and gain great success for the sensitive quantification of biomolecules. However, they sometimes suffer from the inconveniences of time-consuming labeling, low stability of bioconjugates, and the function disorder of labeled-biomolecules. Recently, DNA-templated Cu nanoparticles (NPs) exhibit great potential for label-free fluorescent bioassays, thanks to the advantages of outstanding fluorescent properties, low toxicity, low price, good biocompatibility, and more importantly, instantaneous reaction, and facile integration with nucleic acid-based signal amplification and target-recognition strategies. In this review, we aim to discuss major developments and applications of DNA-CuNPs-based label-free assays for biomolecules. The DNA structures were manipulated for amplified sensing, multimode sensing, and versatile applications including cancer cell analysis, polyacrylamide gel electrophoresis, logic circuit, genotyping etc. Emerging analytical strategies were also discussed in detail. We address the advantages, applicable situations, and limitations of DNA-CuNPs-based label-free strategies and propose suggestions for future developments.

标签策略是主流技术，并且在生物分子的灵敏定量中获得了巨大的成功。然而，它们有时遭受费时的标记，生物缀合物的低稳定性以及标记的生物分子的功能障碍的不便。近年来，DNA模板化的铜纳米颗粒（NPs）具有无标记荧光生物测定的巨大潜力，这要归功于其杰出的荧光特性，低毒性，低价格，良好的生物相容性，更重要的是瞬时反应和与核酸的轻松整合基于酸的信号放大和目标识别策略。在这篇综述中，我们旨在讨论生物分子的基于DNA-CuNPs的无标记测定的主要发展和应用。DNA结构我们对这些信号进行了放大传感，多模式传感以及包括癌细胞分析，聚丙烯酰胺凝胶电泳，逻辑电路，基因分型等在内的多种应用。还详细讨论了新兴的分析策略。我们解决了基于DNA-CuNPs的无标签策略的优势，适用情况和局限性，并为未来的发展提出了建议。