Counting and Sizing of Single Vesicles/Liposomes by Electrochemical Events,ChemElectroChem

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Counting and Sizing of Single Vesicles/Liposomes by Electrochemical Events
ChemElectroChem ( IF 3.5 ) Pub Date : 2018-07-17 , DOI: 10.1002/celc.201800616
Yang Liu _{1,

2} , Cong Xu _{2,

3} , Ping Yu _{2,

3} , Xuwei Chen ₁ , Jianhua Wang ₁ , Lanqun Mao _{2,

3}

Affiliation

The analysis of single entities in a liquid phase by electrochemical events has received much attention in recent years due to innovations and advances in exciting electrochemical methods as well as precise manufacturing techniques. Vesicles, as a special type of entity, play an important role in biological systems. However, the soft character and complex surface chemistry of vesicles present inherent challenges for single‐vesicle analysis. This minireview mainly focuses on the theory and recent progress of single‐vesicle/liposome analysis based on electrochemical events. Three different analytical principles, namely, pore/channel translocation, microelectrode impact and nanopipette collision, are reviewed and expatiated. Not only the capabilities for size determination, vesicle/liposome counting and the electroactive quantification of the contents within vesicles/liposomes but also the specificities of vesicle/liposome or entity‐pore/electrode interactions reflected in translocation or collision events are discussed in detail. Moreover, the advantages and disadvantages of each method of single‐vesicle analysis are discussed in this minireview.

中文翻译：

电化学事件对单个囊泡/脂质体的计数和大小测定

近年来，由于令人兴奋的电化学方法以及精确制造技术的创新和进步，通过电化学事件对液相中的单个实体进行分析已引起了广泛的关注。囊泡作为一种特殊的实体，在生物系统中起着重要的作用。但是，囊泡的柔软特性和复杂的表面化学性质为单囊泡分析带来了固有的挑战。本微型综述主要关注基于电化学事件的单囊泡/脂质体分析的理论和最新进展。审查和阐述了三种不同的分析原理，即孔/通道易位，微电极碰撞和纳米吸管碰撞。不仅具有确定尺寸的功能，详细讨论了囊泡/脂质体的计数以及囊泡/脂质体中内容物的电活性定量，还讨论了在转运或碰撞事件中反映的囊泡/脂质体或实体孔/电极相互作用的特异性。此外，在本微型评论中讨论了每种单囊泡分析方法的优缺点。

更新日期：2018-07-17

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