Acteoside has been reported to have antioxidant and neuroprotective effect, which is a promising therapeutic way in prevention and treatment of Parkinson's disease. The present study was aimed to understand the neuroprotective effect of acteoside and to elucidate its underlying mechanism. 6-hydroxydopamine (6-OHDA)-induced neural damage in zebrafish model was used to study the protective effect of acteoside on Parkinson's disease (PD). Locomotion behavioral test showed that acteoside could prevent 6-OHDA-stimulated movement disorders. Anti-tyrosine hydroxylase (TH) whole-mount immunostaining analysis showed that acteoside could prevent 6-OHDA-induced dopaminergic neuron death. In addition, pretreatment with acteoside could upregulate antioxidative enzymes by activating the Nrf2/ARE signaling pathway in zebrafish. Meanwhile, acteoside was found to be distributed in the brain after intraperitoneal injection into the adult zebrafish, indicating that this compound could penetrate the blood-brain-barrier (BBB). This study demonstrated that acteoside could penetrate BBB and have potential therapeutic value for PD by activating the Nrf2/ARE signaling pathway and attenuating the oxidative stress.

据报道，Acteoside具有抗氧化和神经保护作用，这是预防和治疗帕金森氏病的一种有前途的治疗方法。本研究旨在了解阿糖甙的神经保护作用并阐明其潜在机制。斑马鱼模型中6-羟基多巴胺（6-OHDA）引起的神经损伤被用来研究洋紫苏苷对帕金森氏病（PD）的保护作用。运动行为测试表明，洋紫苏苷可以预防6-OHDA刺激的运动障碍。抗酪氨酸羟化酶（TH）的整体免疫染色分析表明，Acteoside可以预防6-OHDA诱导的多巴胺能神经元死亡。此外，用Acteoside预处理可以通过激活斑马鱼中的Nrf2 / ARE信号通路来上调抗氧化酶。同时，发现腹腔注射腹腔注射成年斑马鱼后会在大脑中分布肌苷。这表明该化合物可以穿透血脑屏障（BBB）。这项研究表明，acteoside可以通过激活Nrf2 / ARE信号通路并减轻氧化应激，从而渗透BBB并具有潜在的PD治疗价值。