Growth factor-free salt-leached silk scaffolds for differentiating endothelial cells†,Journal of Materials Chemistry B

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Growth factor-free salt-leached silk scaffolds for differentiating endothelial cells†
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2018-06-12 00:00:00 , DOI: 10.1039/c8tb01001c
Liying Xiao _{1,

2} , Caihong Zhu ₃ , Zhaozhao Ding _{1,

2} , Shanshan Liu ₄ , Danyu Yao _{1,

2} , Qiang Lu _{1,

2} , David L Kaplan ₅

Affiliation

Recently, controllable kinetic assembly has been introduced into the salt-leaching process with silk proteins to form scaffolds, which achieved improvement in tuning the micro-structural and mechanical properties. Here, more control of the kinetic assembly of silk in the process was integrated into the salt-leaching process, resulting in significant mechanical modification of the scaffolds generated. Both glycerol addition and treatment to concentrate the protein were used to tune the hydrophilic interactions during aqueous solution processing and to reduce beta-sheet formation during the salt-leaching process. These new scaffolds showed gradient changes in the elastic modulus in the range of 0.9 to 7.9 kPa. Bone marrow mesenchymal stem cells grew well and showed endothelial differentiation behavior on the scaffolds with optimized stiffness. These results indicated that the introduction of the silk kinetic assembly provides an additional option for the control of porous silk scaffold properties.

中文翻译：

用于分化内皮细胞的无生长因子盐浸丝支架†

最近，可控动力学组装已被引入丝蛋白的盐浸过程中以形成支架，从而在调节微观结构和机械性能方面取得了改进。在这里，对过程中丝的动力学组装的更多控制被整合到盐浸过程中，导致所生成的支架发生显着的机械改性。添加甘油和浓缩蛋白质的处理均用于调节水溶液加工过程中的亲水相互作用，并减少盐浸过程中的β-折叠形成。这些新支架的弹性模量在 0.9 至 7.9 kPa 范围内呈现梯度变化。骨髓间充质干细胞在硬度优化的支架上生长良好并表现出内皮分化行为。这些结果表明，丝动力学组装的引入为控制多孔丝支架特性提供了额外的选择。

更新日期：2018-06-12

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