Functionalized Quartz Nanopipette for Intracellular Superoxide Sensing: A Tool for Monitoring Reactive Oxygen Species Levels in Single Living Cell,ACS Sensors

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Functionalized Quartz Nanopipette for Intracellular Superoxide Sensing: A Tool for Monitoring Reactive Oxygen Species Levels in Single Living Cell
ACS Sensors ( IF 8.2 ) Pub Date : 2018-06-12 00:00:00 , DOI: 10.1021/acssensors.8b00185
Rıfat Emrah Ozel ₁ , Gonca Bulbul ₁ , Joanna Perez ₁ , Nader Pourmand ₁

Affiliation

Reactive oxygen species (ROS), including superoxide radical anions, are vital components in numerous biological functions, including cell signaling and immune responses. Since ROS react with other biomolecules and oxidize them quickly, it is essential for cells to have superoxide-scavenging enzymes and other regulating enzymes that can catalyze the dismutation of superoxide radical anions into less damaging molecules. Otherwise, ROS overproduction can cause oxidative damage to DNA, proteins, cells, and tissues, damage that is associated with the pathogenesis of a range of neurodegenerative disorders, age-related diseases, and cancer. Understanding the relationship between superoxide and these disorders can help the development of innovative therapies for combating oxidative stress and degeneration of nerve cells. Although methods to quantify ROS already exist, they are indirect, destructive, ambiguous, and/or cannot provide real-time measurements in single cells. In this paper, we report a technique for sensing superoxide radical anions in single living cells using functionalized nanopipettes. These nanopipettes allow us to enter the cell as we measure intracellular ROS concentrations over time. We observed that these devices provide precise real-time measurements that are accurate and not possible to obtain with other conventional techniques.

中文翻译：

用于细胞内超氧化物传感的功能化石英纳米移液器：一种监测单个活细胞中活性氧水平的工具

包括超氧化物自由基阴离子在内的活性氧（ROS）是许多生物学功能（包括细胞信号传导和免疫反应）中的重要组成部分。由于ROS与其他生物分子发生反应并迅速氧化，因此细胞必须具有超氧化物清除酶和其他调节酶，这些酶可以催化超氧化物自由基阴离子转变为破坏性较小的分子。否则，ROS过量产生会导致DNA，蛋白质，细胞和组织的氧化损伤，这种损伤与一系列神经退行性疾病，与年龄有关的疾病和癌症的发病机理有关。了解超氧化物与这些疾病之间的关系可以帮助开发创新的疗法来对抗氧化应激和神经细胞变性。尽管已经存在量化ROS的方法，但它们是间接的，破坏性的，模棱两可的，和/或无法在单个单元格中提供实时测量。在本文中，我们报告了一种使用功能化的纳米移液器感测单个活细胞中超氧自由基阴离子的技术。这些纳米移液器使我们能够随着时间测量细胞内ROS浓度进入细胞。我们观察到，这些设备提供了精确的实时测量结果，这些测量结果是准确的，无法通过其他常规技术获得。这些纳米移液器使我们能够随着时间测量细胞内ROS浓度进入细胞。我们观察到，这些设备提供了精确的实时测量结果，这些测量结果是准确的，无法通过其他常规技术获得。这些纳米移液器使我们能够随着时间测量细胞内ROS浓度进入细胞。我们观察到，这些设备提供了精确的实时测量结果，这些测量结果是准确的，无法通过其他常规技术获得。

更新日期：2018-06-12

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