Protein pores are highly specific in binding to chiral substrates and in catalysing stereospecific reactions, because their active pockets are asymmetric and stereoselective^1,2. Chiral binding materials from molecular-level pores with high specificity have not been achieved because of problems with pore deformation and blocking³. A promising solution is the self-assembly of single sheets where all pores are exposed to the environment, for example as metal–organic frameworks⁴, polymers^5,6 or non-covalent aromatic networks^7,8,9,10, but, typically, the pores are distant from the internal cavities with chirality. Here, we report the synthesis of homochiral porous nanosheets achieved by the 2D self-assembly of non-chiral macrocycles, with open/closed pore switching. Pore chirality is spontaneously induced by a twisted stack of dimeric macrocycles. The porous 2D structures can serve as enantiomer sieving membranes that exclusively capture a single enantiomer in a racemic mixture solution, with uptake capacity greater than 96%. Moreover, the entrapped guests inside the pores can be pumped out by pore closing triggered by external stimuli. This strategy could provide new opportunities for controlled molecule release, as well as for artificial cells.

蛋白质孔在与手性底物结合和催化立体定向反应方面具有很高的特异性，因为它们的活性口袋是不对称的和立体选择性的^1,2。由于孔变形和阻塞³的问题，尚未获得具有高特异性的分子水平孔的手性结合材料。一种有前途的解决方案是将所有孔隙都暴露于环境中的单个薄片进行自组装，例如将其作为金属-有机骨架⁴，聚合物^5,6或非共价芳族网络^7,8,9,10但是，通常情况下，孔与手性相距内腔较远。在这里，我们报告了通过非手性大环化合物的2D自组装实现的同手性多孔纳米片的合成，具有开/关孔转换功能。毛孔手性是由扭曲的二聚体大环化合物堆栈自发诱导的。多孔2D结构可以用作对映体筛分膜，专门捕获外消旋混合物溶液中的单个对映体，吸收能力大于96％。此外，由于外部刺激而导致的毛孔闭合，可以将毛孔内夹杂的客人抽出。这种策略可以为控制分子释放以及为人造细胞提供新的机会。