In this study, we developed a new immunosensor that can achieve an ultralow detection limit and high sensitivity. This new device has an electrokinetic trapping (EKT)-based nanofluidic preconcentrator, which was integrated with oscillating bubble valves, to trap concentrated antigen and immunobeads. During the immunoassay process, oscillating bubbles rapidly grew and acted as control valves and to block the microchannel. Thereafter, the trapped preconcentrated antigen plug and antibody-coated nanobeads were preserved in the region between these two valves. Finally, the antigen concentration was quantitatively analyzed by a real-time measurement of Brownian diffusion of the immunobeads. In this work, the test sample used was C-reactive protein (CRP) which is a risk indicator of coronary heart disease and atherosclerosis.

中文翻译：

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新型热气泡阀集成纳米流体预浓缩器，用于高度灵敏的生物标志物检测

在这项研究中，我们开发了一种新型免疫传感器，可以实现超低检测限和高灵敏度。这种新设备具有基于电动捕获（EKT）的纳米流体预浓缩器，该浓缩器与振荡气泡阀集成在一起，可以捕获浓缩的抗原和免疫珠。在免疫测定过程中，振荡气泡迅速生长并充当控制阀并阻塞微通道。之后，将捕获的预浓缩抗原栓和抗体包被的纳米珠保存在这两个瓣膜之间的区域中。最后，通过实时测量免疫珠的布朗扩散来定量分析抗原浓度。在这项工作中，使用的测试样品是C反应蛋白（CRP），它是冠心病和动脉粥样硬化的危险指标。