Abstract A novel guided tissue regeneration (GTR) membrane with orthogonal aligned fibers in surface layers and random fibers within interlayer was developed by multilayer electrospinning. The mechanical strength of tri-layer membrane was between random and aligned membranes. The promotion of the proliferations of MC3T3-E1 and NIH-3 T3 cells on the tri-layer membrane were observed during monoculture. When cells were cocultured, the tri-layer membrane was effective in impeding MC3T3-E1 and NIH-3 T3 cell infiltration by guiding cells to grow along the direction of the fiber alignment.

中文翻译：

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用于引导组织再生的具有正交排列表面的纤维膜

摘要 通过多层静电纺丝技术开发了一种新型引导组织再生（GTR）膜，其表层纤维呈正交排列，夹层纤维呈随机排列。三层膜的机械强度介于无规膜和排列膜之间。在单培养过程中观察到MC3T3-E1和NIH-3 T3细胞在三层膜上增殖的促进作用。当细胞共培养时，三层膜通过引导细胞沿纤维排列方向生长，有效阻止 MC3T3-E1 和 NIH-3 T3 细胞浸润。