Cannabidiol (CBD) is a non-psychotomimetic compound of the Cannabis sativa that has been used for the treatment of severe epilepsy as well as other diseases of nervous system. However, toxicity studies of CBD have great relevance to guarantee the patients safety. In this context, morphological analyses of zebrafish can contribute to evaluate the teratogenic potential, as well as evaluation of acetylcholinesterase activity and motor activity of zebrafish are valuable tools to verify the neurotoxicity potential. In the present work, we use this methodology to test the toxicity of CBD to zebrafish embryos. No malformation was observed in morphological analysis of embryos exposed to all tested concentrations of CBD. Although, twenty per cent of embryos exposed to maximal dose of CBD (300 μg/L) hatched after 96hpf, while embryos in control solution had already hatched in this period. Embryos exposed to CBD did not show differences in acetylcholinesterase activity, but embryos exposed to CBD 20–300 μg/L were 1.4 up to 1.7-fold more active when compared to the control. Despite that, at 48 hpf, motor activity returned to control values. Our results suggest that the effects observed after CBD exposure are intimately related to CB1 receptor that is present in zebrafish since early stages of development. The present work showed early light effects induced by CBD exposure in concentrations that did not alter biochemical activity.

大麻二酚（CBD）是大麻的非拟精神成分已经用于治疗严重的癫痫病和其他神经系统疾病。然而，对CBD的毒性研究对于保证患者安全性具有重要意义。在这种情况下，斑马鱼的形态分析可有助于评估其致畸潜力，而评估斑马鱼的乙酰胆碱酯酶活性和运动活性是验证神经毒性潜力的宝贵工具。在目前的工作中，我们使用这种方法来测试CBD对斑马鱼胚胎的毒性。在暴露于所有测试浓度的CBD的胚胎的形态分析中未观察到畸形。尽管暴露于最大剂量的CBD（300μg/ L）的胚胎中有20％在96hpf之后孵化，而对照溶液中的胚胎在此期间已经孵化。暴露于CBD的胚没有显示乙酰胆碱酯酶活性的差异，但是与对照相比，暴露于CBD 20-300μg/ L的胚胎的活性高1.4到1.7倍。尽管如此，在48 hpf时，运动活动恢复到控制值。我们的结果表明，自开发早期以来，CBD暴露后观察到的效果与斑马鱼中存在的CB1受体密切相关。目前的工作表明，在不改变生化活性的浓度下，由CBD暴露引起的早期光效应。我们的结果表明，自开发早期以来，CBD暴露后观察到的效果与斑马鱼中存在的CB1受体密切相关。目前的工作表明，在不改变生化活性的浓度下，由CBD暴露引起的早期光效应。我们的结果表明，自开发早期以来，CBD暴露后观察到的效果与斑马鱼中存在的CB1受体密切相关。目前的工作表明，在不改变生化活性的浓度下，由CBD暴露引起的早期光效应。