The response of metallicolous (M) and nonmetallicolous (NM) Alyssum montanum ecotypes to multi-metal stress was investigated under in vitro condition and compared in this study. Shoot cultures were simultaneously treated with 0.7 mM ZnSO₄, 3.0 μM Pb(NO₃)₂ and 16.4 μM CdCl₂ for 8 weeks and evaluated for their morphogenetic and ultrastructural reaction, growth tolerance as well as ability to Zn, Pb, and Cd uptake. Moreover, tissue localization and concentrations of antioxidant compounds were determined in order to elucidate the potential role of ROS-scavenging machinery in plant tolerance to metal toxicity. The results clearly demonstrated that M specimens treated with heavy metals showed less phytotoxic symptoms and low level of lipid peroxidation than reference NM one. The enhanced tolerance of M ecotype resulted from heavy metals detoxification in trichomes and intracellular leaf compartments as well as balanced ROS accumulation. The inactivation of ROS in M plants was based on peroxidase-flavonoid system, while in NM plants such relationship was not detected and amounts of antioxidant enzymes or phenolic compounds was comparable to untreated specimens or decreased significantly. Considering the procumbent growth of such hemicryptophyte which reproduce effectively in the presence of heavy metals but is characterized by low biomass production, it is proposed to exploit M ecotype of A. montanum in revegetation schemes of polluted calamine wastes to provide the prompt stabilization of areas prone to erosion.

在体外条件下研究了金属香（M）和非金属香（A）香雪球生态型对多金属胁迫的响应，并在本研究中进行了比较。芽培养物同时与0.7毫米的ZnSO处理₄，3.0μM的Pb（NO ₃）₂和16.4μM的CdCl ₂持续8周，评估它们的形态发生和超微结构反应，生长耐受性以及对Zn，Pb和Cd吸收的能力。此外，确定了组织的定位和抗氧化剂化合物的浓度，以阐明ROS清除机制在植物对金属毒性的耐受性中的潜在作用。结果清楚地表明，与参考NM-1相比，用重金属处理过的M标本显示出更少的植物毒性症状和低水平的脂质过氧化作用。对M生态型的耐受性增强是由于毛状体和细胞内叶区室中的重金属排毒以及平衡的ROS积累所致。M植物中ROS的失活是基于过氧化物酶-类黄酮系统，而在NM植物中，未检测到这种关系，并且抗氧化酶或酚类化合物的量与未经处理的标本相当或显着降低。考虑到在重金属存在下能有效繁殖但具有低生物量生产特征的这种半植物的繁茂生长，建议利用M型生态系统。A. montanum在受污染的炉甘石废物的植被恢复计划，提供的易受侵蚀地区稳定的提示。