The in vitro effects of individual brominated flame retardants (BFRs), selected metals, and their binary mixtures on ethoxyresorufin-O-deethylase (EROD) activity were evaluated using a plate-reader method. The BFRs, 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47), decabromodiphenyl oxide (BDE-209), hexabromocyclododecane (HBCD), and tetrabromobisphenol A (TBBPA), were tested at doses ranging from 0.1 ng/L to 100 µg/L. Selected metals (Cu²⁺, Cd²⁺, Hg²⁺, and Zn²⁺) were screened at doses of 0.1 mg/L to 50 mg/L. The activity of EROD was significantly induced by TBBPA, BDE-209, and Zn²⁺, while HBCD, Cu²⁺, Cd²⁺, and Hg²⁺ decreased EROD activity. Moreover, following exposure to binary mixtures of metals and BFRs, the EROD activity dose-response curves were similar to those of the metals alone, indicating that EROD activity was governed by the metals.

使用读板法评估了各种溴化阻燃剂（BFR），选定的金属及其二元混合物对乙氧基间苯二酚-O-脱乙基酶（EROD）活性的体外影响。测试BFR，2,2'，4,4'-四溴二苯醚（BDE-47），十溴二苯醚（BDE-209），六溴环十二烷（HBCD）和四溴双酚A（TBBPA）的剂量范围为0.1 ng / L至100 µg / L。以0.1 mg / L至50 mg / L的剂量筛选出选定的金属（Cu 2 ⁺，Cd 2 ⁺，Hg ²⁺和Zn ²⁺）。TBBPA，BDE-209和Zn ²⁺显着诱导EROD活性，而HBCD，Cu 2 ⁺，Cd ²⁺和Hg ²⁺显着诱导EROD活性。EROD活性降低。此外，暴露于金属和BFR的二元混合物后，EROD活性的剂量反应曲线与单独的金属相似，表明EROD活性受金属控制。