In recent years, considerable research effort has explored the interaction between semiconducting minerals and microorganisms, such relationship is a promising way to increase the efficiency of bioelectrochemical systems. Herein, the enhancement of electron transfer between birnessite photoanodes and Pseudomonas aeruginosa PAO1 under visible light was investigated. Under light illumination and positive bias, the light–birnessite–PAO1 electrochemical system generated a photocurrent of 279.57 μA/cm², which is 322% and 170% higher than those in the abiotic control and dead culture, suggesting photoenhanced electrochemical interaction between birnessite and Pseudomonas. The I-t curves presented repeatable responses to light on/off cycles, and multi-conditions analyses indicated that the enhanced photocurrent was attributed to the additional redox species associated with P. aeruginosa PAO1 and with the biofilm on birnessite. Electroconductibility analysis was conducted on the biofilm cellularly by conductive atomic force microscope. Pyocyanin was isolated as the biosynthesized extracellular shuttle and characterized by cyclic voltammetry and surface-enhanced Raman spectroscopy. Rapid bioelectron transfer driven by light was observed. The results suggest new opportunities for designing photo-bioelectronic devices and expanding our understanding of extracellular electron transfer with semiconducting minerals under light in nature environments.

近年来，大量的研究工作探索了半导体矿物与微生物之间的相互作用，这种关系是提高生物电化学系统效率的一种有前途的方式。在此，研究了可见光下水钠锰矿光阳极与铜绿假单胞菌PAO1之间电子转移的增强。在光照和正偏压下，水-水钠锰矿-PAO1电化学系统产生的光电流为279.57μA/ cm ²，比非生物对照和死培养中的光电流分别高322％和170％，这表明水钠锰矿和水合物之间的光增强电化学相互作用假单胞菌。It曲线呈现出对光开/关循环的可重复响应，并且多条件分析表明，增强的光电流归因于与铜绿假单胞菌PAO1和水钠锰矿上的生物膜相关的其他氧化还原物质。通过导电原子力显微镜在生物膜上对生物膜进行导电性分析。绿脓素是作为生物合成的细胞外穿梭物而分离的，并通过循环伏安法和表面增强拉曼光谱进行表征。观察到由光驱动的快速生物电子转移。这些结果为设计光生物电子设备和扩大我们对自然环境中光下半导体矿物与细胞外电子转移的理解提供了新的机会。