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Malic Acid Carbon Dots: From Super-resolution Live-Cell Imaging to Highly Efficient Separation
ACS Nano ( IF 15.8 ) Pub Date : 2018-06-08 00:00:00 , DOI: 10.1021/acsnano.8b01619 Bo Zhi 1 , Yi Cui 2 , Shengyang Wang 1 , Benjamin P. Frank 3 , Denise N. Williams 4 , Richard P. Brown 4 , Eric S. Melby 2 , Robert J. Hamers 5 , Zeev Rosenzweig 4 , D. Howard Fairbrother 3 , Galya Orr 2 , Christy L. Haynes 1
ACS Nano ( IF 15.8 ) Pub Date : 2018-06-08 00:00:00 , DOI: 10.1021/acsnano.8b01619 Bo Zhi 1 , Yi Cui 2 , Shengyang Wang 1 , Benjamin P. Frank 3 , Denise N. Williams 4 , Richard P. Brown 4 , Eric S. Melby 2 , Robert J. Hamers 5 , Zeev Rosenzweig 4 , D. Howard Fairbrother 3 , Galya Orr 2 , Christy L. Haynes 1
Affiliation
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As-synthesized malic acid carbon dots are found to possess photoblinking properties that are outstanding and superior compared to those of conventional dyes. Considering their excellent biocompatibility, malic acid carbon dots are suitable for super-resolution fluorescence localization microscopy under a variety of conditions, as we demonstrate in fixed and live trout gill epithelial cells. In addition, during imaging experiments, the so-called “excitation wavelength-dependent” emission was not observed for individual as-made malic acid carbon dots, which motivated us to develop a time-saving and high-throughput separation technique to isolate malic acid carbon dots into fractions of different particle size distributions using C18 reversed-phase silica gel column chromatography. This post-treatment allowed us to determine how particle size distribution influences the optical properties of malic acid carbon dot fractions, that is, optical band gap energies and photoluminescence behaviors.
中文翻译：
苹果酸碳点：从超高分辨率活细胞成像到高效分离
发现与合成染料相比，合成后的苹果酸碳点具有出色的光闪烁性能。考虑到它们优异的生物相容性，苹果酸碳点适用于多种条件下的超分辨率荧光定位显微镜，如我们在固定和活鳟鱼g上皮细胞中所证明的。此外，在成像实验过程中，未观察到单独的苹果酸碳点的“激发波长相关”发射，这促使我们开发一种省时，高通量的分离技术来分离苹果酸使用C 18将碳点分成不同粒度分布的馏分反相硅胶柱色谱。这种后处理使我们能够确定粒度分布如何影响苹果酸碳点馏分的光学性质，即光学带隙能和光致发光行为。
更新日期：2018-06-08
中文翻译：
苹果酸碳点：从超高分辨率活细胞成像到高效分离
发现与合成染料相比，合成后的苹果酸碳点具有出色的光闪烁性能。考虑到它们优异的生物相容性，苹果酸碳点适用于多种条件下的超分辨率荧光定位显微镜，如我们在固定和活鳟鱼g上皮细胞中所证明的。此外，在成像实验过程中，未观察到单独的苹果酸碳点的“激发波长相关”发射，这促使我们开发一种省时，高通量的分离技术来分离苹果酸使用C 18将碳点分成不同粒度分布的馏分反相硅胶柱色谱。这种后处理使我们能够确定粒度分布如何影响苹果酸碳点馏分的光学性质，即光学带隙能和光致发光行为。
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