Fabrication and Characterization of β-Lactoglobulin-Based Nanocomplexes Composed of Chitosan Oligosaccharides as Vehicles for Delivery of Astaxanthin,Journal of Agricultural and Food Chemistry

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Fabrication and Characterization of β-Lactoglobulin-Based Nanocomplexes Composed of Chitosan Oligosaccharides as Vehicles for Delivery of Astaxanthin
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-06-08 00:00:00 , DOI: 10.1021/acs.jafc.8b00834
Chengzhen Liu ₁ , Zhuzhu Liu ₁ , Xun Sun ₁ , Shuaizhong Zhang ₁ , Shuhui Wang ₂ , Fuxian Feng ₃ , Dongfeng Wang ₁ , Ying Xu ₁

Affiliation

Astaxanthin (Ax), a type of carotenoid, has limited use as a result of its poor water solubility, low bioavailability, and decomposition under harsh conditions. This study reports a delivery system for Ax through a simple affinity binding with β-lactoglobulin and then coated with chitosan oligosaccharides. Ax-loaded β-lactoglobulin nanocomplexes and chitosan oligosaccharide-coated nanocomplexes were successfully prepared. The nanocomplexes exhibited a smooth spherical shape with diameters of about 40 and 60 nm measured by transmission electron microscopy. Spectroscopic techniques (ultraviolet–visible, fluorescence, and Fourier transform infrared spectroscopy) combined with molecular docking were used to determine the binding mechanism of Ax and β-lactoglobulin. In comparison to native Ax, the nanocomplexes maintain the hydroxyl radical scavenging activity of Ax under the treatment of acid, high temperature, and ultraviolet radiation. The release experiment of nanocomplexes revealed that the encapsulation could provide prolonged release of Ax in simulated gastrointestinal juices. This study aimed to fabricate and characterize Ax−β-lactoglobulin nanocomplexes, which can improve the Ax stability and slow release.

中文翻译：

壳聚糖寡糖为载体的虾青素传递载体的基于β-乳球蛋白的纳米复合物的制备与表征

虾青素（Ax）是一种类胡萝卜素，由于其水溶性差，生物利用度低以及在恶劣条件下分解而受到限制。这项研究报告了通过与β-乳球蛋白的简单亲和力结合，然后用壳聚糖寡糖包被的Ax递送系统。成功制备了载有斧头的β-乳球蛋白纳米复合物和壳聚糖寡糖涂层的纳米复合物。纳米复合物表现出光滑的球形，其通过透射电子显微镜测得的直径为约40和60nm。光谱技术（紫外可见，荧光和傅里叶变换红外光谱）结合分子对接技术用于确定Ax与β-乳球蛋白的结合机理。与原生Ax相比，纳米复合物在酸，高温和紫外线辐射的处理下保持了Ax的羟基自由基清除活性。纳米复合物的释放实验表明，包封可以在模拟胃肠道液中延长Ax的释放。这项研究旨在制造和表征Ax-β-乳球蛋白纳米复合物，它可以改善Ax的稳定性和缓慢释放。

更新日期：2018-06-08

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