CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing.,Nature Communications

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CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing.
Nature Communications ( IF 14.7 ) Pub Date : 2018-06-08 , DOI: 10.1038/s41467-018-04651-5
Toon Swings _{1,

2} , David C Marciano ₃ , Benu Atri ₄ , Rachel E Bosserman ₅ , Chen Wang ₃ , Marlies Leysen ₁ , Camille Bonte ₁ , Thomas Schalck _{1,

2} , Ian Furey ₆ , Bram Van den Bergh _{1,

2} , Natalie Verstraeten _{1,

2} , Peter J Christie ₅ , Christophe Herman ₃ , Olivier Lichtarge _{3,

4,

6,

7} , Jan Michiels _{1,

2}

Affiliation

CRISPR advances genome engineering by directing endonuclease sequence specificity with a guide RNA molecule (gRNA). For precisely targeting a gene for modification, each genetic construct requires a unique gRNA. By generating a gRNA against the flippase recognition target (FRT) site, a common genetic element shared by multiple genetic collections, CRISPR-FRT circumvents this design constraint to provide a broad platform for fast, scarless, off-the-shelf genome engineering.

中文翻译：

CRISPR-FRT 以敲除集合中的共享位点为目标，进行现成的基因组编辑。

CRISPR 通过指导 RNA 分子 (gRNA) 引导核酸内切酶序列特异性，推进基因组工程。为了精确靶向基因进行修饰，每个基因构建体都需要独特的 gRNA。通过生成针对翻转酶识别目标 (FRT) 位点（多个遗传集合共享的常见遗传元件）的 gRNA，CRISPR-FRT 规避了这一设计限制，为快速、无疤痕、现成的基因组工程提供了广泛的平台。

更新日期：2018-06-08

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