AbstractPolystyrene nanofibers were coated with copper nanoparticles (CuNPs) by a combination of electrospinning and in-situ reduction of Cu(II) using sodium borohydride as the reductant. The CuNPs on the nanofibers were characterized by energy dispersive spectrometry, scanning electron microscopy and transmission electron microscopy. A cartridge was packed with the nanofibers which then were activated with methanol and water. Glutathione (GSH) is found to quantitatively adsorbed by the packed cartridge at pH 3.0, and then can be desorbed with aqueous 2-mercaptoethanol and detected, after derivatization with ortho-phthalaldehyde, via high performance liquid chromatography with fluorometric detection. Under optimized conditions, the method has a 1.1 ng·mL−1 detection limit and a response that is linear in the 10–1000 ng·mL−1 GSH concentration range. The recoveries of GSH from artificial urine spiked at three levels (80, 400 and 800 ng·mL−1) are in the range of 94.6–98.6% with relative standard deviations (RSD) of <4.5% (n = 5). The method was applied to assessing the differences in urinary GSH between high-risk infants and healthy infants. The results show that the levels of GSH of normal infants are significantly higher than those of high-risk infants (P < 0.05). Graphical abstractSchematic of the preparation of CuNP-assembled nanofibers and the mechanism of extracting glutathione (GSH). GSH can be extracted by this material based on a strong interaction between the sorbent and GSH. This is attributed to the formation of Cu-S bonds between Cu and -SH.

中文翻译：

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用铜纳米粒子包覆的聚苯乙烯纳米纤维用于选择性提取谷胱甘肽，然后用 HPLC 测定

摘要 以硼氢化钠为还原剂，通过静电纺丝和原位还原 Cu(II) 相结合，在聚苯乙烯纳米纤维上包覆了铜纳米粒子 (CuNPs)。通过能量色散光谱、扫描电子显微镜和透射电子显微镜对纳米纤维上的 CuNPs 进行表征。用纳米纤维填充墨盒，然后用甲醇和水活化。发现谷胱甘肽 (GSH) 在 pH 3.0 时被填充柱定量吸附，然后可以用 2-巯基乙醇水溶液解吸，并在用邻苯二醛衍生后通过具有荧光检测的高效液相色谱进行检测。在优化条件下，该方法的检测限为 1.1 ng·mL-1，响应在 10–1000 ng·mL-1 GSH 浓度范围内呈线性。三种浓度（80、400 和 800 ng·mL-1）的人工尿液中 GSH 的回收率在 94.6-98.6% 的范围内，相对标准偏差 (RSD) 小于 4.5% (n = 5)。该方法用于评估高危婴儿与健康婴儿的尿GSH差异。结果显示，正常婴儿GSH水平显着高于高危婴儿（P < 0.05）。图形摘要CuNP组装纳米纤维的制备示意图和提取谷胱甘肽（GSH）的机理。基于吸附剂和 GSH 之间的强相互作用，这种材料可以提取 GSH。这归因于Cu和-SH之间形成Cu-S键。6%，相对标准偏差 (RSD) <4.5% (n = 5)。该方法用于评估高危婴儿与健康婴儿的尿GSH差异。结果表明，正常婴儿GSH水平显着高于高危婴儿（P < 0.05）。图形摘要CuNP组装纳米纤维的制备示意图和提取谷胱甘肽（GSH）的机理。基于吸附剂和 GSH 之间的强相互作用，这种材料可以提取 GSH。这归因于Cu和-SH之间形成Cu-S键。6%，相对标准偏差 (RSD) <4.5% (n = 5)。该方法用于评估高危婴儿与健康婴儿的尿GSH差异。结果表明，正常婴儿GSH水平显着高于高危婴儿（P < 0.05）。图形摘要CuNP组装纳米纤维的制备示意图和提取谷胱甘肽（GSH）的机理。基于吸附剂和 GSH 之间的强相互作用，这种材料可以提取 GSH。这归因于Cu和-SH之间形成Cu-S键。结果表明，正常婴儿GSH水平显着高于高危婴儿（P < 0.05）。图形摘要CuNP组装纳米纤维的制备示意图和提取谷胱甘肽（GSH）的机理。基于吸附剂和 GSH 之间的强相互作用，这种材料可以提取 GSH。这归因于Cu和-SH之间形成Cu-S键。结果表明，正常婴儿GSH水平显着高于高危婴儿（P < 0.05）。图形摘要CuNP组装纳米纤维的制备示意图和提取谷胱甘肽（GSH）的机理。基于吸附剂和 GSH 之间的强相互作用，这种材料可以提取 GSH。这归因于Cu和-SH之间形成Cu-S键。