当前位置: X-MOL 学术J. Funct. Foods › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
L-Carnitine activates calcium signaling in human osteoblasts
Journal of Functional Foods ( IF 3.8 ) Pub Date : 2018-06-07 , DOI: 10.1016/j.jff.2018.05.068
Anita Ferraretto , Michela Bottani , Isabella Villa , Lucia Giusto , Michela Signo , Pamela Senesi , Anna Montesano , Fernanda Vacante , Livio Luzi , Alessandro Rubinacci , Ileana Terruzzi

L-Carnitine (L-C) is a conditionally essential nutrient required for β-oxidation and cell energy production whose unbalanced metabolism is associated with a variety of diseases. L-C increased cell proliferation, differentiation and mineralization of human osteoblast in culture. The present study evaluated the effect of L-C on intracellular calcium signaling, an important modulator of cell proliferation and differentiation in human osteoblasts by means of Fura-2 fluorescence coupled to video imaging technique. 5 mM L-C promoted a calcium influx from the extracellular milieu by depolarizing L-type calcium channels. Moreover, L-C depleted the calcium stores of the mitochondria and of the endoplasmic reticulum through phospholipase C activation. The inhibition of the calcium sensing receptor with 10 µM NPS-2143 prevented the L-C dependent cytosolic calcium increase suggesting that this calcium mobilization is mediated by Calcium Sensing Receptor activation. These results unravel that L-C might be part of a complex nutritional environment which tunes osteoblast activity.



中文翻译:

左旋肉碱激活人成骨细胞中的钙信号

左旋肉碱(LC)是β-氧化和细胞能量产生所需的条件必需营养素,其代谢失衡与多种疾病有关。LC增加了培养物中人成骨细胞的细胞增殖,分化和矿化作用。本研究通过Fura-2荧光和视频成像技术评估了LC对细胞内钙信号传导的影响,钙信号传导是人类成骨细胞中细胞增殖和分化的重要调节剂。5 mM LC通过使L型钙通道去极化来促进钙从细胞外环境中涌​​入。此外,LC通过磷脂酶C的激活耗尽了线粒体和内质网的钙存储。用10 µM NPS-2143抑制钙敏感受体可防止LC依赖性胞质钙增加,表明该钙动员是由钙敏感受体激活介导的。这些结果表明,LC可能是调节成骨细胞活性的复杂营养环境的一部分。

更新日期:2018-06-07
down
wechat
bug