The effect of GPETAFLR, a peptide isolated from Lupinus angustifolius L. protein hydrolysate (LPH), on osteoclastogenesis was investigated. Human osteoclasts generated from monocytes were used to analyse the effects of GPETAFLR (50–100 µg/mL) on osteoclastogenesis using TRAP reaction, RT-qPCR, and ELISA procedures. LPS enhanced TRAP activity and the expression of osteoclast marker genes (TRAP, OSCAR, RANK, and CATHK) while downregulated the expression of OPG gene in human monocyte-derived osteoclasts. These effects were reduced with GPETAFLR. Moreover, LPS increased the release of osteoclastogenic cytokines (TNF-α, IL-1β, and IL-6) meanwhile GPETAFLR increased the release of anti-osteoclastogenic cytokines (IL-4 and IL-10) in the medium of human monocyte-derived osteoclasts. For the first time, we show that plant peptides from lupine protein hydrolysates have anti-osteoclastogenic activity. These exciting findings open opportunities for developing nutritional strategies with Lupinus angustifolius L. as dietary source of plant proteins, notably GPETAFLR, to prevent development and progression of osteoclast-related diseases.

GPETAFLR（从羽扇豆（Lupinus angustifolius）分离的肽）的作用研究了L.蛋白水解物（LPH）对破骨细胞的作用。由单核细胞产生的人类破骨细胞被用于通过TRAP反应，RT-qPCR和ELISA方法分析GPETAFLR（50–100 µg / mL）对破骨细胞的作用。LPS增强了TRAP活性和破骨细胞标记基因（TRAP，OSCAR，RANK和CATHK）的表达，同时下调了人单核细胞衍生破骨细胞中OPG基因的表达。使用GPETAFLR可以降低这些影响。此外，LPS增加了破骨细胞生成的细胞因子（TNF-α，IL-1β和IL-6）的释放，而GPETAFLR增加了人单核细胞衍生培养基中抗破骨细胞生成的细胞因子（IL-4和IL-10）的释放。破骨细胞。首次，我们显示了来自羽扇豆蛋白水解产物的植物肽具有抗破骨细胞活性。羽扇豆作为植物蛋白（特别是GPETAFLR）的饮食来源，可预防破骨细胞相关疾病的发生和发展。