The nanozymes are a kind of synthetic nanomaterials with enzyme-like properties. Metal–organic frameworks (MOFs) are an important class of inorganic–organic hybrid crystals. Their unique composition, structural diversity and size tailorability enable them to be promising for the construction of novel nanozymes. This review intends to summarize the recent advance for the construction of MOFs-based nanozymes and their primary applications in chemical sensing and biosensing. Based on their synthetical strategies, the MOFs-based nanozymes are categorized into four classes: pristine MOFs, MOFs with chemical modification, MOFs-based composites and MOF derivatives. In each categorization, the design and enzyme mimetic activity are discussed. Moreover, the analytical applications of these nanozymes are covered, such as the detection of H₂O₂, small biomolecules releasing H₂O₂, reductive small biomolecules, biomacromolecules, heavy metal ions, toxic metabolite of fungi, antibiotics, and so on. Finally, a summary and future perspective on the applications of MOFs-based nanozymes are briefly discussed.

纳米酶是一种合成的纳米材料具有类似酶的特性。金属-有机骨架（MOF）是无机-有机杂化晶体的重要一类。它们独特的组成，结构多样性和大小可定制性使它们在构建新型纳米酶方面大有前途。这篇综述旨在总结基于MOFs的纳米酶的构建及其在化学传感和生物传感中的主要应用的最新进展。基于其合成策略，基于MOF的纳米酶可分为四类：原始MOF，经过化学修饰的MOF，基于MOF的复合物和MOF衍生物。在每个分类中，都讨论了设计和酶模拟活性。此外，还涵盖了这些纳米酶的分析应用，例如H ₂ O ₂的检测。，释放H ₂ O _2的小生物分子，还原性小生物分子，生物大分子，重金属离子，真菌的有毒代谢产物，抗生素等。最后，简要讨论了基于MOFs的纳米酶的应用概述和未来展望。