Stem cell therapy has great potential for the treatment of ischemic diseases, but poor engraftment of implanted stem cells limits the therapeutic efficacy. Here, we developed an approximately 80 μm injectable decellularized matrix (IDM) to increase the angiogenic efficacy of mesenchymal stem cells by improving the engraftment of the stem cells implanted in to an ischemic tissue. Adhesion of human adipose tissue-derived stem cells (hADSCs) to the IDM enhanced the cell viability and upregulated angiogenic factors in vitro under either cell adhesion-suppressive conditions or hypoxic conditions, which simulated the microenvironment of ischemic tissues. In a murine ischemic-hindlimb model, hADSCs that were attached to the IDM and subsequently injected into an ischemic region showed better grafting and angiogenic factor expression. The hADSC–IDM implantation subsequently promoted the formation of microvessels, attenuated fibrosis, and increased blood perfusion in the ischemic region, as compared to implantation of hADSCs only. The IDM may be an effective off-the-shelf material that can enhance therapeutic efficacy of stem cell therapy for ischemic diseases.

中文翻译：

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一种可注射的脱细胞基质，可改善间充质干细胞移植的治疗性血管生成。

干细胞疗法具有治疗缺血性疾病的巨大潜力，但植入的干细胞植入不良会限制治疗效果。在这里，我们开发了大约80μm的可注射脱细胞基质（IDM），以通过改善植入缺血组织的干细胞的植入来提高间充质干细胞的血管生成功效。人脂肪组织干细胞（hADSCs）与IDM的粘附在体外抑制细胞粘附的条件下或缺氧条件下增强了细胞活力并上调了血管生成因子，从而模拟了缺血组织的微环境。在鼠缺血后肢模型中，附着于IDM并随后注入缺血区域的hADSCs显示出更好的移植和血管生成因子表达。与仅植入hADSC相比，hADSC–IDM植入随后促进了缺血区域的微血管形成，纤维化减弱和血液灌注增加。IDM可能是一种有效的现成材料，可以增强干细胞疗法治疗缺血性疾病的疗效。