The development of a nitric oxide (NO)-generating surface with long-term, stable and controllable NO release improves the therapeutic efficacy of cardiovascular stents. In this work, we developed a “one-pot” method inspired by mussel adhesive proteins for copolymerization of selenocystamine (SeCA) and dopamine (Dopa) to form a NO-generating coating on a 316 L stainless steel (SS) stent. This “one-pot” method is environmentally friendly and easy to popularize, with many advantages including simple manufacturing procedure, high stability and no involvement of organic solvents. Such SeCA/Dopa coatings also enabled us to develop a catalytic surface for local NO-generation by reaction of endogenously existing S-nitrothiol species from fresh blood. We found that the developed SeCA/Dopa coatings could release NO in a controllable and stable manner for more than 60 days. Additionally, the released NO significantly inhibited smooth muscle cell (SMC) proliferation and migration, as well as platelet activation and aggregation through the up-regulation of cyclic guanosine monophosphate synthesis. Moreover, such NO generation enhanced the adhesion, proliferation and migration of endothelial cells (ECs), and achieved rapid in vivo re-endothelialization, effectively reducing in-stent restenosis and neointimal hyperplasia. We envision that the SeCA/Dopa-coated 316 L SS stent could be a promising platform for treatment of cardiovascular diseases.

具有长期，稳定和可控的NO释放的生成一氧化氮（NO）的表面的开发提高了心血管支架的治疗功效。在这项工作中，我们开发了一种受贻贝粘附蛋白启发的“一锅法”，用于硒代半胱胺（SeCA）和多巴胺（Dopa）的共聚反应，从而在316 L不锈钢（SS）支架上形成NO生成涂层。这种“一锅法”对环境友好，易于推广，具有许多优点，包括制造过程简单，稳定性高且不涉及有机溶剂。此类SeCA / Dopa涂层还使我们能够通过来自新鲜血液的内源性存在的S-硝基硫醇物种的反应，为局部NO生成开发催化表面。我们发现，开发的SeCA / Dopa涂层可以60天内以可控且稳定的方式释放NO。另外，释放的NO通过上调环状鸟苷单磷酸的合成而显着抑制平滑肌细胞（SMC）的增殖和迁移，以及血小板的活化和聚集。此外，这种NO的产生增强了内皮细胞（EC）的粘附，增殖和迁移，并实现了快速体内再内皮化，有效减少支架内再狭窄和新内膜增生。我们设想，SeCA / Dopa涂层的316 L SS支架可能是治疗心血管疾病的有前途的平台。